四逆散及其拆方对实验性溃疡性结肠炎大鼠IL-4/STAT6通路的影响

Study on Sini Powders and Different Compatibilities Influence on IL-4 / STAT6 Pathway in Experimental Ulcerative Colitis Rats

目的:从基因水平进一步探讨四逆散调控实验性溃疡性结肠炎大鼠IL-4/STAT6信号通路的作用机制。方法:将实验动物分为6组,采用免疫法造模,以大鼠IL-4、SOCS1、STAT6的基因表达作为观察与检测指标。结果:四逆散组实验大鼠IL-4、SOCS1表达显著增加(P<0.05),而STAT6显著降低(P<0.05);三个拆方组中,柴芍配伍对IL-4、SOCS1 mRNA表达的影响与四逆散无显著性差异(P>0.05),但STAT6 mRNA的表达显著高于正常组(P>0.05);与柴枳甘组、芍枳甘组比较,柴芍组IL-4、SOCS1 mRNA表达明显升高(P>0.05),STAT6 mRNA的表达显著降低(P>0.05)。结论:四逆散能够通过刺激IL-4、SOCS1 mRNA的表达,抑制STAT6mRNA的表达来调节IL-4/STAT6通路,干预实验性UC。方中柴芍配伍作用明显,主要体现在对IL-4、SOCS1mRNA表达的影响上;柴、芍分别配伍柴枳后,对TL-4/STAT6通路的调节作用明显降低。

Objective： To further explore the mechanism of Sini powders regulating IL - 4/STAT6 signal pathway in experimental ulcerative colitis rats at gene level. Methods ： The experimental animals were divided into 6 groups, establishing the model with immune method. We used rats＇IL -4, SOCS1 and STAT6 gene expressions as the observation and detection index. Results：The rats＇IL-4 and SOCS1 expressions of Sini powders group increased significantly （P 〈 0.05 ） while STAT6 decreased significantly （ P 〈 0.05）. Comparing three disassembled prescription groups, Chaishao group had no significant difference when compared with the Sini powders group on expressions of SOCS1 and IL - 4 mRNA （ P 〉 0.05 ） but the expression of STAT6 mRNA was significantly higher than the normal group~ （P 〉 0.05 ）. Compared with Chaizhigan group and Shaozhigan group, the Chaishao group~ IL- 4 and SOCS1 mRNA expressions were significantly in- creased （P 〉 0.05） but the expression of STAT6 mRNA decreased significantly （P 〉 0.05）. Conclusion ：The Sini powders can stimulate expressions of IL -4 and SOCS1 mRNA and inhibit expression of STAT6 mRNA ,and then regulate the IL -4/ STAT6 pathway to intervene the experiment UC. Chaishao compatibility has obvious effects, mainly reflecting in the influence on expressions of IL-4 and SOCS1 mRNA. And the function would be reduced when using the compatibility of Chaizhi.