祛风宣痹方对实验性哮喘豚鼠气道炎症和Th1/Th2细胞因子的影响

Effect of Qufeng Xuanbi Formula on Experimental Asthma Guinea Pigs' Airway Inflammation and Th1/Th2 Cytokines

目的:观察祛风宣痹方对哮喘豚鼠气道炎症和Th1/Th2细胞因子的影响,探讨其作用机制。方法:用卵蛋白和雾化吸入致敏法,复制豚鼠哮喘模型;取肺泡灌洗液进行嗜酸性粒细胞(Eos)计数;肺组织切片HE染色形态学观察;ELASA方法检测BALF中IFN-γ和IL-4的含量。结果:模型组较正常组肺泡灌洗液(BALF)中气道Eos炎症细胞表达异常,组织形态学病变明显,IFN-γ含量显著下降,IL-4的含量明显升高;地米组、中药高剂量组、中药低剂量组较模型组均有显著性差异,BALF中Eos的数量明显降低,组织形态学改善明显,IFN-γ含量显著升高,IL-4的含量明显下降,中药高剂量组效果强于低剂量组。结论:祛风宣痹方能减少EOS浸润,抑制气道炎症,调节Th1/Th2失衡,这可能是其阻断哮喘发生发展的机制之一。

Objective： To explore the therapeut!c effect and mechanism of Qufeng Xuanbi Formula on experimental asthma guinea pigs airway inflammation and regulation Th1/Th2 imbalance in treatment of bronchia asthma. Methods： Animal models were established in guinea pigs by a combination of intraperitional injection and repeated intranasal challenges. Immune adjustment influence of medicine on Thl/Th2 imbalance was observed. Eos in BALF were taken out to count and tissue slice dyed with HE were observed. Contents of IFN -γand IL - 4 were detected by ELASA. Results ：Compared with normal group, the alveolar lavage fluid （BALF） Eos abnormal expression and histomorphology lesions were obviously abnormal;the level of IFN -γ was dramatically decreased and level of IL - 4 was all significantly in- creased in the BALF. DXM group , high and low dose groups had significant difference compared with model group （ P 〈 0.01 ）. However, the percentages of EOS and level of IFN -γ were dramatically increased and the level of IL - 4 was dramatically decreased （P 〈0.01 ）. The high dose group effect is better than that of the low dose group. Conclusion： Qufeng Xuanbi Formula can reduce the EOS infiltration, inhibit airway inflammation and regulate Thl/Th2 imbalance. It could be one of the blocking mechanism of occurrence and development of asthma.