益生菌对急性溃疡性结肠炎大鼠肠黏膜Toll样受体表达的影响

Effects of probiotics on the expression of TLRs in rat models of acute ulcerative colitis

目的探讨益生菌对溃疡性结肠炎(UC)大鼠模型肠黏膜Toll样受体(TLRs)表达的调控及其在UC发生发展中可能的作用机制。方法 40只SD大鼠随机分为正常对照组(A组)、模型组(B组)、金双歧治疗组(C组)、Toll样受体4单克隆抗体(TLR4m Ab)干预组(D组)。采用三硝基苯磺酸(TNBs)法造模,C组给予金双歧灌服,D组给予TLR4m Ab腹腔注射,第8天处死全部大鼠。对每组大鼠进行疾病活动指数(DAI)评分及组织病理学(HPS)评分,应用实时荧光定量(RT-PCR)法测定TLR4、TLR2 m RNA的表达。结果 B组DAI及HPS评分均明显高于A组(P<0.05),C组和D组较模型组均有所缓解(P<0.05)。TLR4、TLR2 m RNA在B组的表达明显高于A组,差异有统计学意义(P<0.05),而在C组和D组的表达差异无统计学意义(P>0.05)。结论 UC大鼠结肠组织中TLR4、TLR2表达异常,益生菌能抑制TLR4、TLR2的表达,可能是一种有效治疗UC的方法。

Objective To explore the effects of probiotics on the expression of TLRs in rat models of ulcerative colitis（ UC） and its possible role in the pathogenesis of UC. Methods 40 SD rats were randomly divided into normal control group（ group A）, model group（ group B）, Jinshuangqi group（ group C） and TLR4 m Ab group（ group D）. Group B,C and D were given trinitrobenzene sulfonic acid（ TNBs） to induce acute intestinal inflammation,group C were given Jinshuangqi by oral gavage,and group D were given TLR4 m Ab intraperitoneal injection; all rats were sacrificed on the 8th day. To evaluate the disease activity index（ DAI） score and colon histopathology score（ HPS）,the expression of TLR4,TLR2 m RNA were measured by real-time fluorescence quantitative（ RT-PCR）. Results The HPS and DAI scores in group B were significantly higher than in group A（ P〈0. 05）.Compared with the group B,group C and D were evidently lower（ P〈0. 05）. The expression of TLR4 and TLR2 m RNA in group B were evidently higher than in group A（ P〈0. 05）,while the differences between the group C and D were not observably significant（ P〉0. 05）. Conclusion The expression of TLR4 and TLR2 in the colonic tissue of UC rats is abnomal. Probiotics can inhibit the expression of TLR4 and TLR2,and may be an effective method for the treatment of UC.