密度梯度离心联合上游法的精液处理在供精体外受精胚胎移植中的应用

Application of Semen Preparation by the Density Gradient Centrifugation Technique Combined with the Swim-Up Method in Vitro Fertilization and Embryo Transfer with Donor Semen

目的:观察供精体外受精-胚胎移植术(D-IVF)中采用密度梯度离心法联合上游法处理精液后的胚胎情况。方法:回顾本院2013年1月-2014年7月D-IVF 135个周期,观察冷冻精液复苏后经采用密度梯度离心法联合上游法处理后精子总活动率、前向运动精子活动率等精液情况,以及受精以后的受精率、卵裂率、临床妊娠率,对照组为同期46个因丈夫梗阻性无精行ICSI的周期。结果:处理后精子总活动率、前向运动精子活动率均大大提高,但是精子密度却大大下降,处理前后比较差异均有统计学意义(P<0.05);受精以后的实验室资料和对照组比较,各项参数差异无统计学意义(P>0.05)。结论:密度梯度离心法联合上游法的精液处理方法在D-IVF中是一种稳定可行的方法。

Objective： To observe embryonic development by using sperm selection prepared by the density gradient centrifugation technique combined with the swim-up method in vitro fertilization and embryo transfer with donor semen （ D-IVF ）. Method： 135 cases of routine D-IVF were retrospective analyzed from January 2013 to July 2014. The sperm condense, total activity rate and progressive motility were observed after sperm recovery and sperm selection. The fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate were compared by the 135 cases and the control groups consist of 46 cases of ICSI （ intracytoplasmic sperm injection ） with husband semen. Result： After treatment, the total activity rate of sperm and the sperm activity rate were significantly increased, but the sperm density was significantly decreased, the differences were statistically significant （P〈0.05 ） .Compared the fertilization rate, cleavage rate, high quality embryo rate and pregnancy rate of two groups, the differences were no statistically significant （ P〉0.05 ） .Conclusion： The density gradient centrifugation technique combined withthe swim-up method is a stable and feasible method in D-IVF.