Simultaneous determination of five major components of Glycyrrhizae Radix et Rhizoma in Xiaoer Zhike Tangjiang with standardized reference extract

应用标准对照提取物同时测定小儿止咳糖浆中甘草的五个主要成分（英文）

In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma （GRR） in Xiaoer Zhike Tangjiang （XEZKTJ） with standardized reference extract （SRE）. The five analytes （liquiritin apioside, liquiritin, isoliquiritin apioside, liquirigenin and glycyrrhizic acid） were well separated with good linearity, precision, stability and repeatability. The recovery rates ranged from 95.69% to 100.80%. The content of the five compounds in 34 batches of commercial XEZKTJ products was determined using standardized GRR extract （SRE method） and individual chemical reference standards （CRS method）. Highly similar results were obtained from the two methods, demonstrating the feasibility of the proposed SRE method. Taken together, we proposed an efficient and low-cost way to perform multi-component quality control of XEZKTJ in this study.

本研究应用标准对照提取物建立了一个简单的HPLC-DAD方法以同时定性、定量测定小儿止咳糖浆中甘草的五个主要成分。应用本方法可使芹糖甘草苷、甘草苷、芹糖异甘草苷、甘草苷元和甘草酸等甘草主要成分得到有效分离,并具有良好的线性、精密度、稳定性与重现性,其加样回收率为95.69%–100.80%。应用本方法对不同来源的34批小儿止咳糖浆样品进行了五个主成分的含量测定,并与应用单独化学对照品的含量测定方法进行了对比性研究与分析,结果表明两种方法获得的测定结果表现出高度的一致性,证明了标准对照提取物测定方法的可行性。本研究建立的方法为小儿止咳糖浆的多成分质量控制提供了简单高效、切实可行的低成本检测方法。