MMP7启动子区基因多态性分布与结直肠癌相关性的研究

Association between gene polymorphism of MMP7 promoter region and colorectal cancer

目的研究MMP7启动子区rs11568818位点多态性与结直肠癌易感性的关系。方法选取哈尔滨医科大学附属第二医院结直肠癌患者80例和健康人80例,抽取外周静脉血,提取基因组DNA,扩增特定基因片段,确定每例标本的MMP7启动子区rs11568818(-181A/G)位点基因型,分析基因型频率及等位基因频率。结果 MMP7启动子区rs11568818位点基因型A/A、A/G和G/G在结直肠癌组例数为17(21.2%)、40(50%)、23(28.8%),在对照组为28(35%)、36(45%)、16(20%),两组差异具有显著性;此位点等位基因-181A和-181G在结直肠癌组个数为74(46%)和86(54%),在对照组为92(58%)和68(42%),两组差异有显著性(P<0.05)。结论 MMP7基因多态性与结直肠癌易感性有关,纯合型-181G是结直肠癌发病的一个高危因素。

Objective To investigate the relationship between MMP7 promoter rs11568818 polymorphism and susceptibility of colorectal cancer（ RCR）. Methods Genomic DNA samples were isolated from peripheral blood of 80 RCR patients that were collected at The Second Affiliated Hospital of Harbin Medical University and 80 blood donors who were in a control group of healthy. Polymerase chain reaction-restriction fragment length polymorphism methodology was applied to identify the genotypes of MMP7 promoter rs11568818（- 181 A / G） locus in each specimens. Frequencies of genotype and allele status were analyzed based on the Gene Counting Method. Results MMP7 promoter rs11568818 locus genotype A / A,A / G and G / G were 17（ 21. 2%）,40（ 50%）,23（ 28. 8%） in CRC group,while 28（ 35%）,36（ 45%）,16（ 20%） in controls,and there was a significance difference between them. The allele- 181 A and- 181 G were 74（ 46%） and 86（ 54%） in CRC patients,while 92（ 58%） and 68（ 42%）in control group,and the difference was significant（ P〈0. 05）. Conclusion MMP7- 181 G polymorphism is associated with the susceptibility to CRC,and the GG genotype is a risk factor for the cause of colorectal cancer.