结直肠癌K-ras基因突变两种检测方法的比较及临床相关性分析

Comparison of two methods for the detection of K-ras mutations in colorectal cancer and correlation with clinical characteristics

目的比较ARMS法和Sanger测序法在检测K-ras基因突变时的敏感性、一致性和特异性,并且探讨结直肠癌患者K-ras基因突变与临床病理特性的相关性。方法采用ARMS法和Sanger测序法对200例结直肠癌患者进行K-ras基因突变检测。结果 ARMS法检出K-ras基因突变75例,阳性率为37.50%。Sanger测序法检出K-ras基因突变72例,阳性率为36.00%。两种方法的符合率为96%。同时,K-ras基因突变与淋巴结转移具有相关性(P<0.05),与患者年龄、性别、肿瘤部位、分化程度、肝转移和肿瘤类型均无相关性(P>0.05)。结论 K-ras基因突变检测有助于筛选靶向治疗有效的结直肠癌患者。ARMS法更适用于临床检测。

Objective To compare the ARMS method and Sanger sequencing method in detection of K-ras gene mutation of sensitivity,consistency and specificity,and to explore the relationship between K-ras gene mutation and the clinical pathological characteristics. Methods In 200 cases of colorectal cancer,K-ras gene mutations were detected by ARMS method and Sanger sequencing method. Results K-ras gene mutation was found in 75 cases by ARMS method,the positive rate was 37. 50%,while 72 cases by Sanger sequencing method,which positive rate was 36%. The coincidence rate of the two methods was 96%. At the same time,the mutation of K-ras gene had correlation with lymph node metastasis（ P〈0. 05）,and had no correlation with patient age,gender,tumor location,differentiation degree,liver metastasis and tumor type（ P〉0. 05）. Conclusion Detection of K-ras gene mutation contributes to the selection of targeted and effective in the treatment of patients with colorectal cancer. The ARMS method is more suitable for clinical detection.