应用基因芯片技术检测四种结核药物敏感试验的研究

Application of Gene Chip Technology to Detect the Four kinds of TB Research of Drug Sensitive Test

目的探讨基因芯片技术在结核病临床快速诊断中的应用价值。方法应用基因芯片技术对74例临床标本进行链霉素、乙胺丁醇、异烟肼和利福平耐药检测,以罗氏(L-J)绝对浓度法药敏试验为金标准,对基因芯片技术的检测效果进行评价。结果有39例结果完全符合,符合率为52.7%;基因芯片技术检测链霉素、异烟肼、利福平和乙胺丁醇的结核菌药物敏感试验的敏感性分别为91.2%、57.7%、87.3%和78.1%,特异性均为100%,其中异烟肼和乙胺丁醇结果的差异有统计学意义。结论应用基因芯片技术检测四种抗结核药物敏感性试验,可弥补传统药敏试验方法耗时长的不足,为临床使用链霉素、乙胺丁醇、利福平和异烟肼提供预见性用药建议,可与罗氏金标准方法互为补充检测。

Objective To explore the application value of gene chip technology in the rapid diagnosis of tuberculosis in clinic. Methods The application of gene chip technology in clinical specimens of 74 cases of streptomycin , ethambutol , isoniazid and ri- fampin resistance detection by L - J absolute concentration method, Roche drug sensitivity test as a gold standard, to evaluate the effect of gene chip detection technology. Results 39 cases results in full compliance, the coincidence rate was 52.7% ; the sensitivi- ty of gene chip technology to detect streptomycin , isoniazid, rifampicin and ethambutol tuberculosis drug sensitivity test were respec- tively 91.2%, 57.7%, 87.3% and 78.1%, the specificity was 100%, the difference was statistically significant among the isoniazid and ethambutol results. Conclusion The four kinds of anti tuberculosis drug susceptibility test by gene chip technique, which can make up the traditional test method of drug sensitivity of time - consuming shortage, streptomycin, ethambutol, isoniazid and rifampin provide predictability recommendations for clinical use, and L -J goldstandard method of complementary detection..