目标miRNAs在口腔白斑癌变患者唾液中的表达研究

Expression of mi RNAs in patients' saliva with oral leukoplakia

目的:寻找能够准确评估口腔白斑癌变风险的生物标志物指导临床工作。方法:随机收集未经治疗的口腔癌患者、稳定白斑、癌变白斑患者各20例,收集唾液标本,采用RT-q PCR探讨癌变口腔白斑患者唾液mi RNA的变化。结果:癌变白斑患者唾液中mi R-339-5p、mi R-144、mi R--521-3p、mi R-429和mi R-181a与病变组织中的表达情况一致(P<0.10)。结论:mi RNA表达改变的唾液检测有望成为一种理想的、无创的早期检测癌变口腔白斑的新方法。

Objective：Clinical and histological assessments are not reliable in predicting which precursor lesions will progress.The aim of this study was to assess the potential of a noninvasive approach to assess progress risk of oral precancerous lesions.Method：We used RT-q PCR to detect mi RNA targets from the microarray results in saliva samples of these patients.Result：We identified a specific mi RNA signature that is aberrantly expressed in progressing oral LGD leukoplakias.Similar expression patterns were detected in saliva samples from these patients.Conclusion：These results show promise for using saliva mi RNA signature for monitoring of cancer precursor lesions and early detection of disease progression.