高剂量维生素K_2对大鼠主动脉钙化的影响

Effect of vitamin K_2 on calcification in rat aorta in vivo

目的研究高剂量维生素K2(Vit K2)对华法令(Warfarin,WFN)诱导大鼠主动脉钙化的影响。方法将30只SD大鼠随机分为正常对照组、6周(W)钙化组、6W钙化+6W正常饲料组、12W钙化组及6W钙化+6W Vit K2组。用Von Kossa染色法检测胸主动脉组织中钙结节,测定颈总动脉组织中的钙沉积含量及检测血清中碱性磷酸酶的活性。结果 WFN干预12周期间动脉中钙沉积含量呈线性增加(P<0.01)。6W钙化+6W Vit K2组钙沉积含量与6W钙化组相比减少39%(与12W钙化组相比减少60%,P<0.01),治疗后主动脉钙化约40%的预成型的钙盐被移除。6W钙化+6W Vit K2组碱性磷酸酶的活性与6W钙化组、12W钙化组比较均明显降低(P<0.01)。结论高剂量的Vit K2对大鼠主动脉钙化具有抑制和(或)逆转的作用。

AIM To study the effect of high intake of vitamin K2 on the calcification in rat aorta in vivo. METHODS Rats were divided into normal group （n=6, normal diet） and 6-week calcification group （n=6, 3.0 and 1.5 mg W＆K1/g for 6 weeks） and 6 weeks calcification＋6 weeks normal group （n=6, 3.0 ＆ 1.5 mg W＆K1/g for 6 weeks and normal diet for another 6 weeks） and 12-week calcification group （n=6, 3.0 ＆ 1.5 mg W＆K1/g for 12 weeks） and 6 weeks calcification＋6 weeks vitamin K2 group （n=6, 3.0 ＆ 1.5 mg W＆K1/g for 6 weeks and K2 100 μg/g for another 6 weeks）. Alizarin red S and Von Kossa staining was applied to count the calcium nodes and the aorta calcium depositions were measured. RESULTS Continuation of warfarin-containing diet for 12 weeks led to an almost linear increase of the arterial calcium content. High-vitamin K2 intake not only blocked the progress of further calcium accumulation but also led to 〉39% reduction of previously accumulated arterial calcium precipitates within 6 weeks （60% as compared with what at the 12-week calcification time point, P〈0.01）. After a 6-week period （weeks 7-12）, 40% of the preformed calcium salts had been removed. Compared with those in the calcification group, count of calcium nodes, aorta calcium depositions, alkaline phosphatase activity were reduced in vitamin K2 group （P〈0.01）. CONCLUSION High intake of vitamin K2 inhibits and/or reverses the calcification in rat aorta in vivo.