炎症性肠病患者外周血白细胞介素12家族细胞因子的表达及临床意义

Expression and significance of interleukin-12 family cytokines in peripheral blood of patients with inflammatory bowel disease

目的:探讨白细胞介素12(IL-12)家族细胞因子包括IL-12、IL-23、IL-27及IL-35在炎症性肠病(inflammatory bowel disease,IBD)患者血清中的表达情况及其意义。方法:采集105例确诊为IBD患者的外周血(其中克罗恩病组65例,溃疡性结肠炎组40例),另采集24例肠息肉患者的外周血作为对照组,利用酶联免疫吸附法检测3组患者的血清中IL-12、IL-23、IL-27及IL-35的含量,比较3组表达水平差异并分析其与IBD间的关系。结果:溃疡性结肠炎组患者血清中IL-12、IL-27的表达显著高于对照组(P<0.05),而克罗恩病组患者血清中IL-12、IL-23、IL-27以及IL-35表达均显著高于对照组(P<0.05);相关性分析显示IL-12家族细胞因子血清表达水平均与IBD发病相关,IL-12、IL-27及IL-35血清表达水平与溃疡性结肠炎的发病密切相关;IL-12血清表达水平与克罗恩病发病的严重程度呈正相关(P=0.042,r=0.145),而其他细胞因子与克罗恩病、溃疡性结肠炎疾病严重程度间均无关联。结论:IL-12、IL-23、IL-27及IL-35在IBD患者外周血中高表达,且其发病与IL-12家族细胞因子的表达上调密切相关,但表达水平与IBD疾病严重程度间则无统计学关联。

Objective： To investigate the expression and significance of interleukin-12 family cytokines（IL-12, IL-23,IL-27 and IL-35） in patients with inflammatory bowel disease. Methods： One hundred and five patients with inflammatory bowel disease（65 cases of Crohn＇s disease,40 cases of ulcerative colitis） were enrolled and 24 cases of colon polyps were served as controls. Expression levels of serum IL-12, IL-23, IL-27 and IL-35 were detected by enzyme-linked immunosorbent assay. Relationship between expression levels of IL-12, IL-23, IL-27, IL-35 and pathogenesis of inflammatory bowel disease were analyzed. Results： Levels of serum IL-12 and IL-27 in ulcerative colitis and levels of serum IL-12, IL-23, IL-27 and IL-35 in Crohn′s disease were significantly higher than those of control group（P0.05）. Correlation analysis showed that serum levels of IL-12 family cytokines were associated with the pathogenesis of IBD. Serum levels of IL-12, IL-27,and IL-35 were closely related with the pathogenesis of ulcerative colitis, IL-12 had positive correlation with severity of CD（P=0.042, r=0.145）, while other factors didn′t have correlations. Conclusions： Levels of serum IL-12, IL-23, IL-27 and IL-35 are increased in patients with IBD and they might play an important role in IBD. However, they have no obvious correlation with the activity of disease.