孕穗期低温对寒地水稻叶片保护酶活性的影响

Effects of Low Temperature on Protective Enzyme Activities of Rice Leaf during Booting Stage in Cold Area

通过人工控温,对水稻品种空育131叶片在孕穗期遭受低温胁迫后的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的酶活性和丙二醛（MDA）含量进行了研究。结果表明,3种保护酶（SOD、CAT、POD）活性随着处理温度的降低呈升高的变化趋势,随着处理时间的延长呈先升高（1-3 d）后下降（3-5 d）的变化趋势;SOD和CAT酶活性随着处理后恢复生长时间的延长而呈下降的变化趋势,而POD酶活性则相反;丙二醛（MDA）含量受低温胁迫后上升,随着处理温度的降低和处理时间延长呈再升高的变化趋势,随着处理后恢复生长时间的延长而呈逐渐降低的变化趋势。因此处理温度越低,持续时间越长,叶片受害越严重;受害温度高、时间短的处理在保护酶的作用下可基本恢复正常生长。

Analysis was conducted on the activity of peroxidase（POD）, catalase（CAT） and superoxide dismutase（SOD） and Malondialdehyde（MDA） content in the leaves of rice variety Kongyu 131 after low temperature treatments. The results showed that the activity of protective enzymes（SOD, CAT, POD） of the leaves increased with the decrease of temperature, the activity of the three kinds of protective enzyme increased and then decreased with the extension of treatment time. The activity of SOD and CAT showed a decline trend with the increase of recovery time, but POD enzyme activity showed opposite trend. Malondialdehyde（MDA） content increased under the low temperature treatment during booting stage, and increased with the decrease of temperature and increase of treatment time, and decreased with the increase of recovery time. The lower temperature and longer duration treatment made a severe influence on rice leaf. Rice leaf treated with shorter duration in the higher temperature can basically returned to normal growth under the action of protective enzyme.