隔药饼灸对功能性消化不良肝郁脾虚模型大鼠海马盐皮质激素受体、糖皮质激素受体、5-羟色胺1A受体表达的影响

Effect of cake-separated moxibustion on expression of mineralocorticoid receptor,glucocorticoid receptor and 5-hydroxytryptamine receptor 1A in the hippocampus of functional dyspepsia rats with liver-stagnation and spleendeficiency syndrome

目的:观察隔药饼灸对功能性消化不良(functional dyspepsia,FD)肝郁脾虚模型大鼠海马盐皮质激素受体(mineralocorticoid receptor,MR)、糖皮质激素受体(glucocorticoid receptor,GR)、5-羟色胺1A受体(5-hydroxytryptamine receptor 1A,5-HTR1A)表达的影响.方法:将50只SD大鼠按随机数字表法随机分为空白组、模型组、隔药饼灸组、逍遥散组、多潘立酮组5组,每组10只,空白组正常喂养,其余4组采用复合病因造模法(慢性束缚应激+过度疲劳+饮食失节+夹尾+摇晃)造模,连续3 wk;再按被试因素予以隔药饼灸和逍遥散、多潘立酮灌胃,共治疗2 wk.实验结束后采用营养性半固体糊灌胃法测定大鼠胃排空率,用实时荧光定量PCR(real-time fluorescence quantitative PCR,RT-PCR)法检测大鼠海马MR、GR、5-HTR1A的表达.结果:(1)模型组大鼠胃排空率较空白组明显下降(P<0.01),治疗后各治疗组大鼠胃排空率较模型组上升(均P<0.01),但3组间比较差异无统计学意义(P>0.05);(2)与空白组比较,模型组大鼠MR表达差异无统计意义(P>0.05);而G R及5-H T R1A的表达下降且差异均有统计意义(均P<0.05);与模型组比较,隔药饼灸组、逍遥散组、多潘立酮组大鼠海马MR的表达亦无统计意义(P>0.05),而GR及5-HTR1A的表达上调且差异均有统计学意义(均P<0.05),但3组间比较差异均无统计学意义(P>0.05).结论:隔药饼灸同逍遥散及多潘立酮一样能调节海马GR、5-HTR1A的表达,从而调节边缘系统-下丘脑-垂体-肾上腺轴(limbic system-hypothalamic-pituitary-adrenal axis,LHPA/HPA轴),提高大鼠胃排空率,改善胃肠动力,最终起到治疗本病的作用.

AIM：To observe the effect of cake-separated moxibustion on the expression of mineralocorticoid receptor（MR）,glucocorticoid receptor（GR） and5-hydroxytryptamine receptor 1A（5-HTR1A）in the hippocampus of rats with functional dyspepsia（FD）,and to explore the possible mechanism underlying the therapeutic effect of cake-separated moxibustion on FD.METHODS：Fifty SD rats were randomly divided into five groups：a blank group（A）,a model group（B）,a cake-separated moxibustion group（C）,a Xiaoyaosan group（D） and a domperidone group（E）,with 10 rats in each group.Except group A,the other four groups received chronic restraint stress ＋ excessive fatigue ＋ irregular food for 3 wk.After molding,group C received herbal cake-separated moxibustion for 2 wk,and groups D and E were given Xiaoyaosan and domperidone by gavage for the same duration,respectively.At the end of the experiment,gastric emptying was observed and the expression of MR,GR and5-HTR1 A in the hippocampus was measured by real-time fluorescence quantitative PCR（RTPCT）.RESULTS：The rate of gastric emptying in model rats was significantly lower than that of group A（P 0.01）,while the rates of gastric emptying in the three treatment groups were significantly higher than that in group B（P 0.01 for all）,although there were no significant differences among the three treatment groups（P 0.05）.The expression of MR in the hippocampus in group B was statistically alike to that in group A（P 0.05）,while the expression of GR and 5-HTR1 A was significantly lower than that in group A（P 0.05 for both）.Compared with group B,the expression of MR in the hippocampus in groups C,D and E was statistically similar（P 0.05）,but the expression of GR and 5-HTR1 A was significantly higher（P 0.05 for all）.There were no significant differences in the expression of GR or 5-HTR1 A among the three treatment groups（P 0.05）.CONCLUSION：Cake-separated moxibustion can regulate the expression of GR and 5-HTR1 A in the hippocampus to accommodate the function of the limbic-hypothalamic-pituitary-adrenal axis to increase the gastric emptying rate of FD rats,and to improve the gastrointestinal motility as Xiaoyaosan and domperidone.This might be one of the mechanisms underlying the therapeutic effect of cake-separated moxibustion on FD.