摘要
目的探讨莱菔硫烷(SFN)是否通过核因子相关因子2-抗氧化反应元件(Nrf2-ARE)通路对抗大鼠心脏移植冷缺血再灌注损伤。方法健康雄性Sprague-Dawley(SD)大鼠40只随机分成3组:假手术组(Sham组,n=8),缺血再灌注组(I/R组,n=16),莱菔硫烷预处理组(I/R+SFN组,n=16)。建立大鼠异体异位心脏移植模型,将冷藏于4℃HTK液9h的供心移植到I/R组和I/R+SFN组受体大鼠腹腔,移植后24h取出移植心脏。Sham组开/关腹24h后取出自体心脏。应用HE染色法、免疫组织化学法及western blot方法观察心肌组织学、核因子相关因子2(Nrf2)、血红素加氧酶1(HO-1)、醌氧化还原酶1(NQO1)的蛋白表达水平。结果与I/R组比较,I/R+SFN组心肌组织损伤减轻,心肌组织Nrf2核蛋白、Nrf2、HO-1和NQ01蛋白水平显著升高(P〈0.01)。与Sham组比较,I/R+SFN组心肌组织学和心肌组织Nrf2核蛋白、Nrf2、HO-1和NQO1蛋白水平仍高于正常(P〈0.01)。结论莱菔硫烷通过激活Nrf2-ARE通路,上调下游蛋白HO-1、NQO1表达,提高心肌细胞对氧化应激的防御能力,对抗移植心脏冷缺血再灌注损伤。
Objective To discuss whether sulforaphane (SFN) can reduce cold ischemia-reperfusion injury through Nrf2-ARE pathway in rats' heart transplantation. Methods 40 health Male Sprague-Dawley(SD) rats were randomly divided into 3 groups: sham group : n = 8, I/R group--ischemia reperfusion group, n = 16, I/R + SFN group-sulforaphane preconditioning group, n = 16. Dounor rats' hearts stored in 4℃ HTK solution for 9 hours were transplanted into recipient rats intraperitoneal, and then rat heterotopic heart transplantation model was performed in I/R and I/R + SFN groups. Autologous heart and graft were take out 24 hours after heart transplantation in sham group, IRI group, and IRI + SFN group. Cardiac histology and nuclear factor-related factor 2 ( Nrf2 ) , heine oxygenase 1 ( HO-1 ) and quinone oxidoreductase 1 ( NQOI ) protein were observed using hematoxylin-eosin staining, immunohistochemistry and western blot method in each group. Results I/R pretreatment group myocardial tissue damage mitigation, myocardial tissue Nrf2, HO-1, NQO1 protein levels were significantly increased( P 〈0.01 ) compared with ischemia reperfusion group. However, myoeardial histology and myocardial tissue Nrf2 nuclear protein, Nrf2, HO-1 and NQO1 protein levels still higher than normal( P 〈 0.01 ) compared with sham group. Conclusion Sulforaphane improves myocardial cells against oxidative stress defense capability from heart transplantation cold ischemia-reperfusion injury by activating Nrf2-ARE pathway regulated downstream protein HO-1, NQO1 expression.
出处
《中华胸心血管外科杂志》
CSCD
2015年第7期419-421,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
基金
留学回国人员科研启动基金(教外司留20101561)
关键词
莱菔硫烷
心脏移植
再灌注损伤
核因子相关因子2
Sulforaphane Reperfusion injury Nuclear factor-erythroid2-related factor 2 Heine oxygenase-1