二硫化碳染毒对ApoE基因敲除小鼠和C57BL／6J小鼠脂肪酸代谢的影响

目的研究二硫化碳（CS2）染毒对ApoE基因敲除小鼠和C57BL／6J小鼠脂肪酸代谢的影响。方法将24只雄性ApoE基因敲除小鼠随机分为CS2染毒正常饮食组、CS2未染毒正常饮食组、CS2染毒高脂饮食组、CS2未染毒高脂饮食组；24只C57BL／6J雄性小鼠也按同样的方式分成4组；每组6只。将染毒组以浓度为1g／m^3的CS2进行静式吸入染毒，5h／d，5d／周，共2周。收集小鼠全血，采用酸催化甲酯化方法对脂肪酸进行衍生化，并用气质联用（GC-MS）方法比较染毒前后脂肪酸含量。结果C57BL／6J小鼠染毒高脂饮食组花生酸含量明显低于C57BL／6J小鼠未染毒高脂饮食组，ApoE基因敲除小鼠染毒正常饮食组花生四烯酸含量明显低于ApoE基因敲除小鼠未染毒正常饮食组，ApoE基因敲除小鼠染毒高脂饮食组γ-亚麻酸含量明显高于ApoE基因敲除小鼠未染毒高脂饮食组，差异均有统计学意义（P〈0．05）。结论CS2染毒可以引起小鼠脂肪酸代谢紊乱，CS2可能对动脉粥样硬化等心血管疾病有影响。

Objective To study the influences of carbon disulfide (CS2) exposure on fatty acid metabolism in apolipoprotein E (ApoE) knockout mice and C57BL/6J mice.Methods Twenty-four male ApoE knockout mice were randomly and equally divided into four groups:a CS2-exposed normal diet group,a CS2-unexposed normal diet group,a CSrexposed high-fat diet group,and a CS2-unexposed high-fat diet group.Twenty-four C57BL/6J male mice were divided into four groups in the same way.The CS2-exposed groups were exposed to CS2 (1 g/m3) by static inhalation for 5 hours a day,5 days a week.After two weeks,the whole blood of mice was collected.Methyl ester derivatization of fatty acids was performed using an acid-catalyzed method.Fatty acid contents before and after exposure were compared by gas chromatography-mass spectroscopy.Results There were significant differences in fatty acid contents of mice between the four groups.For the C57BL/6J mice,the arachidic acid contents in the CS2exposed high-fat diet group were significantly lower than those in the CS2-unexposed high-fat diet group (P=0.045 0).For the ApoE knockout mice,the arachidonic acid contents in the CS2-exposed normal diet group were significantly lower than those in the CS2-unexposed control diet group (P=0.045 2).For the ApoE knockout mice,the γ-linolenic acid contents in the CS2-exposed high-fat diet group were significantly higher than those in the unexposed high-fat diet group (P=0.044 7).Conclusion Exposure to CS2 can induce fatty acid metabolism disorder in mice,indicating that CS2 may increase the risk of atherosclerosis and other cardiovascular diseases.