黄渤海海域口虾蛄野生资源的种群遗传学研究

Population Genetics of Oratosquilla oratoria in Bohai Sea and Yellow Sea

本研究通过扩增测序共获得了148条口虾蛄线粒体COⅠ基因序列,定义了52个单元型,其中存在63个变异位点。在黄渤海海域中,盐城口虾蛄种群的遗传多样性最高;渤海与黄海海域口虾蛄群体间遗传分化指数（Fst）为0.006 12。经MEGA 4.0软件计算得知11个种群间的遗传距离为0.0009-0.0057,而渤海与黄海两大海域间的遗传距离为0.0043。系统发生关系显示,渤海与黄海海域的单倍型呈交错聚集状态,说明两大海域间不存在显著的遗传分化。本次研究初步评估了两个海域的口虾蛄种群的遗传多样性水平,为渤海海域与黄海海域口虾蛄种质资源保护提供了基础资料。

148 COⅠ sequences of Oratosquilla oratoria were obtained by PCR in this study. A total of 52 haplotypes and63 variable sites were identified in 580 sites of 148 sequences. Of all the 11 populations,the highest genetic diversity was observed in Yancheng population according to the average haplotype diversity and nucleotide diversity. And the genetic differentiation coefficient（ Fst） between Bohai Sea and Yellow Sea was 0. 006 12. The genetic distance among the 11 populations ranged from 0. 0009 to 0. 0057 as determined by MEGA 4. 0 software. However,the average value of genetic distance between Bohai Sea and Yellow Sea was 0. 0043. Phylogenetic tree showed that the 52 haplotypes were not clustered according to different populations,but overlapped with each other. In conclusion,there was no significant genetic differentiation between Bohai Sea and Yellow Sea. These results would be helpful for the conservation of germplasm resources of O. oratoria.