原发性肝癌肿瘤标志物化学发光酶免疫分析方法在临床中的应用

The clinical application of chemiluminescence enzyme immunoassay for primary hepatocellular carcinoma tumor markers

目的探讨原发性肝癌肿瘤标志物化学发光酶免疫分析法在临床中的应用价值。方法选取2013年2—8月收治的肝癌患者100例及正常体检者100例,将肝癌患者作为观察组,正常体检者作为对照组。两组患者进入院后,分别检测AFU(血清α-L-岩藻糖苷酶)、AFP(甲胎蛋白)、CHE(胆碱酯酶)、GGT(γ-谷氨酰基转移酶)、SF(铁蛋白)等指标,并进行对比分析。计量资料采用t检验,计数资料采用χ2检验,P<0.05为差异有统计学意义。结果观察组AFU、AFP、CHE、SF分别为(52.00±0.20)、(32.00±1.20)、(45.25±1.10)×103U/L、(252.00±1.20)μg/L,对照组分别为(32.00±1.20)、(18.00±0.10)、(5.25±1.10)×103U/L、(198.20±0.20)μg/L,两组比较差异均有意义(t=164.399、116.264、257.130、442.233,均P<0.05)。观察组AFU、AFP、CHE、SF检测阳性率分别为8.00%、44.00%、54.00%、57.00%,对照组分别为43.00%、70.00%、85.00%、87.00%,两组比较差异均有统计学意义(χ2=32.241、13.792、22.668、22.321,均P<0.05)。结论检测肿瘤标志物化学发光酶免疫分析法对原发性肿瘤的临床诊断与分析中起到至关重要的作用,扮演着必不可少的角色,具有重要的临床应用价值与可行性,值得推广应用。

Objective To investigate the clinical value of chemical luminescence enzyme immunoassay for tumor markers of primary hepatocellular carcinoma. Methods 100 patients with liver cancer were treated in our hospital from February to August in 2013 as the observation group,and 100 normal persons were selected as the control group. AFU,AFP,CHE,GGT,SF and other indicators were detected in the two groups. Measurement data was processed by t test,count data was processed by chi square test,the result of P 〈0. 05 was considered statistically significant. Results AFU,AFP,Che,SF were（52. 00 ± 0. 20）,（32. 00 ± 1. 20）,（45. 25 ± 21. 10） × 10^3 U / L,（2. 52 ± 1. 20） g / L in the observation group,and（32. 00 ± 1. 20）,（18. 00 ± 0. 10）,（ 5. 25 ± 1. 10） × 10^3 U / L,（ 198. 20 ± 0. 20） g / L in the control group,the differences were significant（ t = 164. 399,116. 264,257. 130,442. 233,all P〈 0. 05）. Of AFU,AFP,Che,SF detection positive rate were 8. 00%,44. 00%,54. 00% and 57. 00% in the Observation group,while 43. 00%,70. 00%,85. 00% and 87% in the control group,the differences were statistically significant（ χ^2= 32. 241,13. 792,22. 668,22.321,all P 〈0. 05）. Conclusions The chemiluminescence enzyme immunoassay method play a crucial role in the diagnosis and analysis of primary tumor. It has important clinical application value and feasibility,and it is worthy of popularization and application conclusions detection of tumor signs.