TNF-α抑制剂对重症急性胰腺炎大鼠凝血功能及钙离子的影响

Effects of TNF-inhibitors on coagulation function and calcium ion in rats with severe acute pancreatitis

目的分析TNF-α抑制剂对重症急性胰腺炎(SAP)大鼠凝血功能及钙离子的影响,为TNF-α抑制剂治疗SAP提供实验依据。方法制做SAP大鼠模型;对照组:假注射组,20只,制备成SAP模型,尾部注射生理盐水;治疗组:SAP模型大鼠60只,治疗组1、治疗组2、治疗组3三个亚组各20只,模型制作成功后,分别按照0.15 mg/kg、0.30 mg/kg、0.45 mg/kg剂量水平,经阴茎背静脉注射TNF-α抑制剂;24 h后,麻醉,采血针抽取各组大鼠颈总动脉血3 m L,行凝血系列检测及观察血钙水平。结果试验期间,大鼠均存活,治疗组大鼠状态稍好,部分仍有自主活动、进食;治疗组1、治疗组2、治疗组3的APPT、FIB水平低于假注射组,治疗组血清离子钙水平高于假注射组;治疗组内3组间PT、APPT、FIB、血清离子钙比较差异具有统计学意义(P<0.05),治疗组内3组间血清总钙比较差异无统计学意义(P>0.05)。结论 SAP可致大鼠凝血功能减退,血钙水平降低,TNF-α抑制剂有助于改善SAP模型大鼠凝血功能及血清离子钙水平,作用机制与抑制炎症反应有关,且呈较明显的剂量依赖,在0.45 mg/kg剂量水平,改善效果更显著。

Objective To investigate the effect of TNF-α inhibitor on coagulation function in a model of rats with severe acute pancreatitis（SAP）. Methods SAP rat model was made. The control group rats（sham-injected group, n =20） were prepared to be made into SAP model with a small amount of saline injection. Sixty rats for SAP model ： 20 rats for therapy group 1, group 2, group 3respectively. After the model was successfully made, TNF-α inhibitor was injected into SAP model through dorsal vena of penis by dose levels of 0.15 mg / kg, 0.30 mg / kg and 0.45 mg / kg respectively.After 24 hrs, anesthesia was performed, and 3 ml common carotid artery blood of rats in all groups were extracted for the tests of coagulation and calcium related. Results During the test, all rats survived. Rats in therapy group were in better status, some of them still had autonomic activities and feeding. APPT and FIB level in therapy group 1, group 2 and group 3 were lower than that of shaminjected group, and comparative differences of PT, APPT, FIB, serum calcium among three therapy groups have statistical significance（P〉0.05）. Between the 3 groups of serum total calcium difference within treatment groups was not statistically significant（P〈0.05）. Conclusion SAP can cause declined coagulation function of rats and serum calcium level decreased. TNF- α inhibitor helps to improve the rats＇ coagulation function of SAP model, and the mechanism of action is related to fluid infusion and inhibiting inflammatory reaction and a quite significant dose-dependent is represented.