IMPDH2蛋白与前列腺癌临床相关性分析

Expression and clinical significances of IMPDH2 protein in prostate cancer tissues and benign prostate hyperplasia tissues

目的研究IMPDH2在良性前列腺增生和前列腺癌中的表达,并分析其表达水平与临床病理特征的关系。方法收集临床手术切除的前列腺癌组织和前列腺增生,或者穿刺活检组织,所有组织均由病理学确诊。排除已做手术去势的前列腺组织。通过Western Blot检测IMPDH2在前列腺癌、前列腺增生组织中IMPDH2蛋白的表达情况;免疫组化检测前列腺癌、前列腺增生标本中IMPDH2的表达。分析IMPDH2基因的表达水平与临床病理特征的关系。结果前列腺癌患者组织中IMPDH2蛋白表达显著上调,IMPDH2蛋白表达上调与肿瘤临床分期、Gleason评分、转移相关。结论 IMPDH2在前列腺癌组织中表达上调,前列腺组织中检测IMPDH2可能有助于判断前列腺癌分化程度并评估预后。

Objective To study the expression and significances of IMPDH2 in benign prostate hyperplasia（BPH） tissues and prostate cancer tissues, and to illustrate the correlation with clinical significance. Methods The samples were derived from benign prostatic tissue and prostatic carcinoma tissue of the patients with BPH or prostatic cancer. Western Blot was used to detect IMPDH2 protein in prostate cancer tissue and BPH tissue. Immunohistochemical method was used to detect IMPDH2 in prostate cancer tissue and BPH tissue,and evaluate the correlations with clinical significances of prostate cancer. Results The expression of IMPDH2 protein was significantly increased in prostate cancer tissues. The expression rate of IMPDH2 was in relation with clinical stage, Gleason score, and metastasis. Conclusion Our study showed the expression of IMPDH2 protein has positive correlation changed trend, suggesting IMPDH2 protein detection may help judge pathologic grade and predict the prognosis of prostate cancer.