乙醇性脂肪肝模型大鼠早期生长反应因子1表达及水飞蓟素的干预

Expression of early growth response factor 1 and intervention of silymarin in rat models of alcoholic fatty liver

背景:促炎性转录因子以及炎性因子在乙醇性肝病的发生中起着重要作用,而早期生长反应因子1正是炎症启动的关键因子之一。目的:观察并分析水飞蓟素对乙醇性脂肪肝模型大鼠早期生长反应因子1的影响。方法:将大鼠采用高脂饮食联合乙醇灌胃方式建立脂肪肝动物模型,每次灌胃后分别给予水飞蓟素高、低剂量(200,100 mg/kg)干预,并设正常组进行对照,共灌胃8周。结果与结论:血清学指标检测和苏木精-伊红染色结果显示,与模型组相比,水飞蓟素高剂量组大鼠体质量增高(P<0.05),血清谷草转氨酶、谷丙转氨酶活性、肝组织早期生长反应因子1和肿瘤坏死因子α的表达降低(P<0.05),病理分级结果均占优(P<0.01)。结果证实,高剂量水飞蓟素能有效保护大鼠肝功能,减少肝功能损害的发生,可能与水飞蓟素抑制大鼠体内早期生长反应因子1,从而减少肿瘤坏死因子α的形成有关,但具体机制尚待进一步研究。

BACKGROUND： Proinflammatory transcription factors and inflammatory factors play an important role in the occurrence of alcoholic liver disease. However, early growth response factor 1 is one of the key factors of starting inflammation. OBJECTIVE： To investigate and analyze the effects of silymarin on early growth response factor1 in rat models of alcoholic fatty liver. METHODS： The rat models were established using the methods of feeding with high fat diet and intragastrically administering alcohol, in total 8 weeks. Silymarin intervention at high and low doses（200,100 mg/kg） was given after each gavage. The normal group was set as comparison. RESULTS AND CONCLUSION： Serological indicator detection and hematoxylin-eosin staining results showed that compared with the model group, the body weight of rats was increased（P〈0.05）; serum aspartate aminotransferase, alanine aminotransferase activity, early growth response factor 1 and tumor necrosis factor-α expression in the liver tissue were decreased（P〈0.05）; pathological grading results were superior in the high-dose silymarin group to in the other groups（P〈0.01）. The results confirm that high dose of silymarin can protect the liver function of rats, reduce the occurrence of liver function damage, which may be associated with the inhibition on the early growth response factor 1 in the body of rats, thereby reducing the tumor necrosis factor-α formation, but the specific mechanism remains to be further studied.