不同加工方法对何首乌饮片质量的影响

Effect of Different Processing Methods on Quality of Polygoni Multiflori Radix Decoction

目的:研究不同加工方法对何首乌饮片中二苯乙烯苷和蒽醌类成分的影响,为何首乌饮片适宜加工方法的建立提供依据。方法:采用不同切片厚度、不同干燥方式和温度处理新鲜何首乌药材,采用薄层色谱法对不同加工方法的何首乌饮片进行定性分析;以二苯乙烯苷和蒽醌类成分为指标,通过含量测定分析不同加工方法对何首乌饮片质量的影响。结果:薄层色谱特征斑点明显。不同干燥温度和干燥方式对何首乌各有效成分的含量影响较大,综合分析以晒干样品的含量最高。不同切片厚度对何首乌有效成分的含量有一定影响,6 mm厚片含量略高于3 mm薄片。结论:不同加工方法对何首乌有效成分的含量影响较大,本文为提高何首乌饮片的质量控制方法提供了一定的依据,对于保证其质量和临床疗效具有重要意义。

Objective： To study the effect of different processing methods on stilbene glycoside and anthraquinones in Polygoni Multiflori Radix（ PMR） decoction,in order to provide the basis for establishing suitable processing methods for PMR decoction. Method： Different slice thicknesses,drying methods and temperatures were adopted to process PMR samples. TLC method was adopted to qualitatively analyze PMR. Stilbene glycoside and anthraquinones were determined by HPLC to define the effect of different processing methods on the quality of PMR decoction. Result： TLC showed obvious spot characteristics. Different drying temperatures and drying methods had a greater impact on the content of effective components in PMR. Samples dried in the sun had the highest content according to the comprehensive analysis. Different slice thicknesses had a certain effect on the content of effective components; 6 mm slices contained more than 3 mm slices. Conclusion： Different processing methods had a greater impact on the content of effective components in PMR. In this paper,the authors provided a certain basis to improve the quality control of PMR,which is significant to ensure PMR’s quality and clinical efficiency.