气相色谱法测定榆叶合叶子中水杨醛的含量

Determination of Salicylaldehyde in Filipendula ulmari by Gas Chromatography

目的：建立榆叶合叶子中水杨醛含量的气相色谱测定方法,为制定榆叶合叶子质量标准提供参考。方法：采用气相色谱法,RTX-1701色谱柱（0.25 mm×30 m,0.25μm）,FID监测器,检测器和进样口温度250℃;升温程序以70℃为起始温度,保持10 min,以1℃·min-1的速率升温至105℃,保持1 min,再以15℃·min-1的速率升温至250℃,保持15 min;载气氮气,流速1 m L·min-1,进样方式分流进样,分流比80∶1,进样量1μL。结果：水杨醛在0.191 7~1.917 0 g·L-1线性关系良好,r=0.999 9,平均回收率100.12%（RSD 2.5%）。结论：该方法简便、准确且重复性好,可作为榆叶合叶子中水杨醛的含量测定方法。

Objective： To establish a method to determine the salicylaldehyde in Filipendula ulmari by gas chromatography（ GC）. Method： By using GC,RTX-1701 column（ 0. 25 mm × 30 m,0. 25 μm） was analyzed with FID detector. The detector and sample inlet temperature were set at 250 ℃. The temperature programming began at 70 ℃ and increased to 105 ℃ at the rate of 1 ℃·min-1with in 10 min and then to 250 ℃at the rate of 15 ℃ within 15 min. Nitrogen was the carrier gas and flew at the rate of 1 m L·min-1. Split sampling was adopted,with the split ratio of 80 ∶ 1 and sample size of 1 μL. Result： The linear ranges for salicylaldehyde were 0. 191 7-1. 917 0 g ·L-1（ r = 0. 999 9）,with the average recovery of 100. 12%（ RSD 2. 5%）.Conclusion： The method is simple,accurate and highly reliable and so can be used for the determination of salicylaldehyde in F. ulmari.