金果榄质量评价

Quality Evaluation of Tinosporae Radix

目的:建立评价金果榄药材质量的方法。方法:采用HPLC,Agilent ZORBX SB-C18色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.5%磷酸水,梯度洗脱,流速1 m L·min-1,检测波长280 nm,进样量10μL,建立不同产地、不同品种金果榄的HPLC指纹图谱,并测定其不同品种中盐酸药根碱与盐酸巴马汀的含量。结果:金果榄药材不同品种的HPLC指纹图谱和盐酸药根碱与盐酸巴马汀的含量存在差异,从金果榄生物碱类成分的指纹图谱中确定了8个共有峰,不同产地的金果榄在生物碱类成分上有差异。相似度大的指纹图谱,表征其含量的指标R不一定大;相似度低的,R不一定小。R的变化趋势与指标成分含量测定结果趋势基本一致。结论:本研究建立的指标成分含量测定方法和指纹图谱的精密度、稳定性、重复性好,可用于表征金果榄质量的差异性,为金果榄的质量研究提供参考依据。

Objective： To establish a method to evaluate the quality of Tinosporae Radix. Method： By using the HPLC method,the Agilent ZORBXSB-C18column（ 4. 6 mm × 150 mm,5 μm） was adopted and eluted in gradient with the mobile phase of acetonitrile-water（ 0. 5% phosphoricacid）. The flow rate was 1 m L·min-1,the detection wave length was 280 nm,and the injection volume was 10 μL. HPLC fingerprints of different varieties of Tinosporae Radix from different producing areas were established to determine the content of jatrorrhizine hydrochloride and martin hydrochloride in different varieties. Result： There were significant differences between different varieties of Tinosporae Radix in their HPLC fingerprints and contents of jatrorrhizine hydrochloride and martin hydrochloride. The fingerprints for alkaloid constituents in Tinosporae Radix showed eight common peaks,with differences in the alkaloid components in Tinosporae Radix from different producing areas. The index R indicating the content may not high in fingerprints with a high similarity. The index R may not low in fingerprints with a low similarity. The index R changed with the index component content determination results. Conclusion：The index component content determination method and fingerprint established in this study was highly precision,stable and repeatable,and can be used to represent the differences in the quality of Tinosporae Radix and provide a reference basis for the quality study on Tinosporae Radix.