赶黄草花与茎、叶HPLC指纹图谱比较

Comparison of HPLC Fingerprint Analysis of Flos, Caulis and Folium from Penthor chinense

目的：建立赶黄草花的HPLC指纹图谱,并对赶黄草花、茎、叶不同部位的指纹图谱进行比较,寻找赶黄草不同部位质量评价与控制方法。方法：采用ODS柱,流动相乙腈-0.1%甲酸水,梯度洗脱,流速0.8 m L·min-1,柱温30℃,检测波长280 nm,进样量10μL。结果：赶黄草花共标示出15个共有峰,其中5号峰为芦丁峰,9号峰为槲皮素峰;10批赶黄草花的相似度在0.925~0.960。赶黄草花分别与茎、叶对比,花的共有峰数目最多,茎最少;茎、叶、花指纹特征峰相对含量有较大差异,花所含特征峰成分的相对含量基本高于茎和叶。结论：该方法准确可靠,重复性好,为赶黄草各部位药材质量评价与控制提供参考;为保证药材质量,建议赶黄草药材在花期采收。

Objective： To establish a method for analysis of Penthorum chinense flos by HPLC fingerprint and compare the fingerprints of caulis,folium and flos,and to seek ways to the quality assessment and quality control of different parts of P. chinense. Method： The analysis was performed on an ODS column with acetonitrile-0. 1% formic acid as mobile phase in gradient elution mode. The flow rate was 0. 8 m L·min-1,the column temperature was 30 ℃ and the injection volume was 10 μL with the detection wavelength of 280 nm.Result： Fifteen common peaks were separated on HPLC fingerprint of the flos of P. chinense,including the fifth peak of rutin peak,the ninth peak of quercetin peaks. The similarities of 10 batches of samples were in the range of 0. 925-0. 960. When the flos of P. chinense was compared with caulis and folium,we found that flos has the most common peaks and caulis has the least. Besides,significant variability was found among the caulis,folium and flos,the contents of marker peak in the flos were more than that in the caulis and folium. Conclusion： The method showed good accuracy, validity and reproducibility characteristics and provided information for the quality assessment and quality control of different parts of P. chinense. To ensure the quality of Chinese medicine,it is recommended that P. chinense should be harvested during florescence.