基于化学成分相互作用的槐花-地榆配伍

Compatibility Based on Interaction of Multiple Components for Sophorae Flos and Sanguisorbae Radix

目的:基于化学成分相互作用,探讨地榆-槐花的配伍作用。方法:设计地榆-槐花药材不同配伍比例,经过提取后,采用紫外分光光度法,分别测定不同比例提取液中总黄酮和鞣质含量;采用体外法测定各提取液清除DPPH自由基能力,并分析DPPH清除能力与各提取液中总黄酮和鞣质含量的关系;同时制备各提取液HPLC指纹图谱,运用统计学方法分析比较配伍后指纹图谱的变化,优选地榆-槐花配伍比。结果:地榆-槐花不同配伍比其总黄酮和鞣质含量,以及DPPH清除率均有差异,其中地榆-槐花在2∶1,1∶1比例下,DPPH清除率较大,总黄酮和总鞣质含量综合得分最高,提示DPPH清除率与黄酮和鞣质含量正相关;不同配伍比,提取液HPLC指纹图谱均有差异,提示配伍对化学成分有一定的影响。结论:地榆-槐花在配伍比例2∶1或1∶1下,其总黄酮、鞣质含量较高,DPPH清除率较大。本实验结果可为地榆-槐花在临床上配伍使用提供依据。

Objective： Study the compatibility based on the interaction of multiple components for Sanguisorbae Radix and Sophorae Flos,and to optimize the excellent compatibility proportion. Method： Different compatibility proportion of Sanguisorbae Radix and Sophorae Flos were designed,and then extract them with hot water, the contents of total flavonoids and total tannins in each extract were determined using ultraviolet spectrophotometric method,and the DPPH radical scavenging activity was used to evaluate the antioxidant capacity,and then analyzed the relationship between DPPH radical scavenging activity and the content of total flavonoids and total tannins. Meanwhile,the HPLC fingerprints of different extracts were tested and comparative analyzed the difference between them. Result： The contents of total flavonoids and total tannins of each extracts were different,and the DPPH scavenging activity was also different. When the compatibility proportion of Sanguisorbae RadixSophorae Flos was 2 ∶ 1 and 1 ∶ 1,the DPPH scavenging activity was better,and the comprehensive score of the contents of total flavonoids and total tannins was the highest,which suggested that the DPPH scavenging activity was positively related to the contents of total flavones and total tannins. And the fingerprints of different extracts were different,which indicated that the compatibility proportion had some influences on chemical components.Conclusion： The contents of total flavonoids and total tannins were higher,and the DPPH scavenging activity was better than others at the compatibility proportion was 2 ∶ 1 or 1 ∶ 1 of Sanguisorbae Radix and Sophorae Flos. The experimental results can be used as a consideration of the compatibility of Sanguisorbae Radix and Sophorae Flos in clinical.