丹参口服液抗心肌缺血活性的作用

Effect of Danshen Oral Solution Against Myocardial Ischemia

目的:探讨丹参口服液的抗心肌缺血活性作用。方法:取SD大鼠70只,随机分为7组,每组10只,即正常组,模型组,阿司匹林组(50 mg·kg-1),复方丹参滴丸组(135 mg·kg-1),丹参片组(300 mg·kg-1),丹参口服液高、低剂量组(5,2.5 g·kg-1),ig给予相应剂量药物7 d,末次给药1 h后,其中正常组股静脉注射生理盐水5 m L·kg-1,其余各组注射10%高分子右旋糖苷(5 m L·kg-1)造成血瘀大鼠模型;另取SD大鼠70只,随机分为7组,每组10只,即正常组,模型组,单硝酸异山梨酯(4 mg·kg-1),复方丹参滴丸组(135 mg·kg-1),丹参片组(300 mg·kg-1),丹参口服液高、低剂量组(生药5,2.5 g·kg-1),ig给予相应剂量药物,末次给药1 h后正常组舌下静脉推注生理盐水,其余各组舌下静脉快速推注垂体后叶素0.5 U·kg-1(1 U·m L-1)造成心肌缺血大鼠模型,采用血液黏度计测定血瘀模型大鼠全血比黏度、血浆比黏度;记录心肌缺血模型大鼠不同时间点Ⅱ导联心电图(ECG)观察T波变化,并测定血浆磷酸肌酸激酶(CK),丙二醛(MDA)及心肌组织超氧化物歧化酶(SOD),钙离子(Ca2+)和MDA。结果:与正常组比较,模型组大鼠全血黏度和血浆黏度明显升高(P<0.01),模型组心肌缺血大鼠不同时间点T波明显升高(P<0.05,P<0.01),心律明显升高,大鼠血浆MDA及CK水平明显升高(P<0.01),心肌组织MDA及Ca2+含量明显升高(P<0.01),心肌组织SOD水平明显降低(P<0.05);与模型组比较,丹参口服液高、低剂量组均可明显降低血瘀大鼠全血黏度和血浆黏度(P<0.05,P<0.01),可明显降低心肌缺血大鼠的T波和心率(P<0.01),明显降低血浆MDA及CK水平(P<0.05,P<0.01);明显降低大鼠心肌组织MDA及Ca2+含量(P<0.05,P<0.01),显著升高SOD水平(P<0.05)。结论:丹参口服液对血瘀模型大鼠和心肌缺血模型大鼠具有活血化瘀及保护的作用。

Objective： To study the anti-myocardial ischemia effect of Danshen oral solution. Method：Totally 70 SD rats were randomly divided into seven groups,with 10 rats in each group. Specifically,they were the control group,the model control group,the Aspirin group（ 50 mg·kg-1）,the compound Danshen dripping pills group（ 135 mg·kg-1）,the Danshen tablets group（ 300 mg·kg-1）,Danshen oral solution high and low dose groups（ 5,2. 5 g·kg-1）. They were orally administered with corresponding drugs for 7 days. One hour later after the last administration,the normal group was injected with physiological saline（ 5 m L·kg-1） through femoral veins,while the other groups were included into the blood stasis model by being injected with 10% high molecular dextran（ 5 m L·kg-1）. Another 70 rats were randomly divided into seven groups,with 10 rats in each group.Specifically,they were the control group,the model control group,the Isosorbide mononitrate group（ 4 mg·kg-1）,the compound Danshen dripping pills group（ 135 mg·kg-1）,the Danshen tablets group（ 300 mg·kg-1）,Danshen oral solution high and low dose groups（ 5,2. 5 g·kg-1）. They were orally administered with corresponding drugs for 7 days. One hour later after the last administration,the normal control group was sublingually injected with normal saline,while the other groups were included into the myocardial ischemia model rats by being sublingually injected with pituitrin 0. 5 U·kg-1（ 1 U·m L-1）. The blood and plasma viscosities of rats in the blood stasis model were detected by blood viscometer. The changes of T wave in electrocardiogram（ ECG） at different time points of rats in the blood stasis model were recorded,and creatine kinase（ CK） and malonyldialdehyde（ MDA） in plasma,superoxide dismutase（ SOD）,Ca2 ＋and MDA in plasma were determined. Result： Compared with the normal group,the blood and plasma viscosities of the model group increased significantly（ P 〈 0. 01）,T wave at different time points in model rats increased significantly（ P 〈 0. 05,P 〈 0. 01）,heart rate,MDA and CK in plasma of model rats increased significantly（ P 〈 0. 01）,MDA and Ca2 ＋content in myocardial tissues of model rats increased significantly（ P 〈 0. 01）,SOD in myocardial tissues of model rats decreased significantly（ P 〈 0. 05）.Compared with the model group,Danshen oral solution high and low dose groups significantly reduce the blood and plasma viscosities in blood stasis rats（ P 〈 0. 05,P 〈 0. 01） and T wave and heart rate in myocardial ischemia rats（ P 〈 0. 01）,MDA,CK in plasma（ P 〈 0. 05,P 〈 0. 01） and MDA and Ca2 ＋content in myocardial tissues of rats（ P 〈 0. 05,P 〈 0. 01） and significantly increase SOD（ P 〈 0. 05）. Conclusion： Danshen oral solution has the protective effect in promoting circulation and removing blood stasis among blood stasis model rats and myocardial ischemia rats.