蝙蝠葛酚性碱对人胰腺癌荷瘤裸鼠移植瘤形态学的研究

Morphology Study of PAMD on Human Pancreatic Cancer Xenografts in Nude Mice

目的:观察蝙蝠葛酚性碱(PAMD)对人胰腺癌细胞BxPC-3裸鼠移植瘤的抑制作用及其病理形态学影响。方法:雌性的裸鼠40只,被接种人源性的胰腺癌细胞株BxPC-3于裸鼠的右腋皮下,主要用于建立人胰腺癌皮下异位移植瘤的实验动物模型。24 h后用随机法将其分为5组,每组8只,分别为PAMD高、低剂量组(20,10 mg·kg-1)5-氟尿嘧啶(5-FU)组(20mg·kg-1),模型组和正常组。PAMD与5-Fu组ip给药,模型组与正常组则给予同体积的生理盐水,但正常组不接种瘤株,给药20 d后,按照相关的公式计算其抑瘤率;在光镜和透射电镜下,观察PAMD对移植瘤组织病理形态学的影响。结果:与正常组比较,模型组瘤重明显增加(P<0.01),肿瘤组织的病理形态和超微结构瘤细胞胞体变大,核浆比例失调,病理性核分裂象增大,核浆比例增大;与模型组比较,PAMD高、低剂量组和5-Fu组对胰腺癌BxPC-3荷瘤裸鼠的瘤组织生长都有一定的抑制作用(P<0.05),其抑瘤率分别为51.67%,37.26%,33.58%,通过光镜和透射电镜显示,PAMD对肿瘤组织的病理形态和超微结构均有一定的改善作用。结论:PAMD能够有效的抑制胰腺癌细胞株Bx PC-3荷瘤裸鼠肿瘤细胞的生长,PAMD高、低剂量组对Bx PC-3荷瘤裸鼠瘤组织的病理形态学变化,具有一定的改善作用。

Objective： To observe the anti-tumor effect and pathological morphology change of phenolic alkaloids of menispermum dauricum（ PAMD） in pancreatic cancer cell line BxPC-3 transplanted into nude mice.Method： The 40 metastatic tumor model of tumor-bearing mice was established by injecting Bx PC-3 cell line hypodermically at right oxter. After 24 h,the mice were randomly divided into 5 groups： the normal group（ normal saline）,the model group（ normal saline）,the 5-FU group（ 20 mg·kg-1）,the high-and low-dose PAMD groups（ 20,10 mg·kg-1） of 8 mice each. The mice except the normal group received heterotopic tumor transplantation of pancreatic carcinoma cell Bx PC-3 subcutaneously. The corresponding medicines were intraperitoneally administered to mice for 20 days. Histomorphology changes of transplanted tumor tissue were observed by using HE staining. The ultrastructure of areas was observed by using electronicmicroscopy. Result： Compared with the normal group the tumor growth of tumor-bearing mice increased,pathological morphology and ultrastructure had obvious changes in the model group（ P 〈 0. 01）. Compared with the model group,the tumor growth inhibited,the inhibition rates were 51. 67%,37. 26%,33. 58%,respectively,in the high-,low-dose PAMD and 5-FU groups（ P 〈 0. 05）.Moreover,pathological morphology and ultrastructure had good improvement. Conclusion： PAMD could effectively suppress tumor-bearing nude mice tumor cell growth of pancreatic cancer cell line BxPC-3 and improve the morphology of transplanted tumor tissue.