天葵子各提取部位体外抗氧化活性及成分研究

Antioxidative Properties and Components of Different Extraction Fractions from Semiaquilegiae Radix in vitro

目的:研究天葵子各提取部位体外抗氧化活性以及主要成分。方法:将天葵子醇提物依次用乙酸乙酯和正丁醇萃取,分别得到乙酸乙酯层(TK-Y)正丁醇层(TK-Z)和水层(TK-S),其中水层通过醇沉法得到水层多糖(TK-D),并以维生素C(VC)为对照,通过Fenton法和邻苯三酚法测定天葵子各部位和水层中的多糖对羟基自由基(·OH)和超氧自由基(O-2·)的清除能力,研究天葵子的抗氧化活性,并以此为导向分离主要活性成分。结果:天葵子抗氧化活性顺序为TK-D>TK-S>TK-Z>TK-Y,其中TK-D对超氧自由基的清除率与浓度呈正相关(Y=54.94X+27.15,R2=0.998 3),IC50为0.42 g·L-1;从正丁醇中分离得到5个化合物,分别鉴定为紫草氰苷(1),东方唐松草苷(2),格列风内酯(3),β-谷甾醇(4),胡萝卜苷(5)。结论:首次证实了天葵子提取物中水层和正丁醇层是其抗氧化活性部位,其中水层中的抗氧化活性物质为多糖,化合物紫草氰苷,东方唐松草苷,格列风内酯是天葵子的主要化学成分。

Objective： To study the antioxidant activity of different fractions and chemical constituents from Semiaquilegiae Radix in vitro. Method： Different extraction fractions from Semiaquilegiae Radix were ethyl acetate extraction（ TK-Y）,n-butanol extraction（ TK-Z）,water extraction（ TK-S） and polysaccharide（ TKD）. Under the chemical simulation conditions in vitro,the antioxidant activities of different extraction fractions and polysaccharide were determined by ·OH,O-2· scavenging experiments. Furthermore,the chemical compounds were isolated from bio-active fraction. Result： The antioxidant activities of different extraction fractions from strong to weak were as follows： TK-D 〉 TK-S 〉 TK-Z 〉 TK-Y. Scavenging activity of TK-D for O-2· and·OH were positively correlated（ Y = 54. 94 X ＋ 27. 15,R2= 0. 998 3）,IC50= 0. 42 g·L-1. Five major components were isolated and identified from TK-Z as lithtospermoside（ 1）,thalictricoside（ 2）,griffonilide（ 3）,β-sitosterol（ 4）,daucosterol（ 5）. Conclusion： The polysaccharide,n-butanol extraction and water extraction are active fractions and it identified the active fractions of Semiaquilegiae Radix for the first time. Lithtospermoside,thalictricoside and griffonilide were found as main contribution.