摘要
目的:研究天葵子各提取部位体外抗氧化活性以及主要成分。方法:将天葵子醇提物依次用乙酸乙酯和正丁醇萃取,分别得到乙酸乙酯层(TK-Y)正丁醇层(TK-Z)和水层(TK-S),其中水层通过醇沉法得到水层多糖(TK-D),并以维生素C(VC)为对照,通过Fenton法和邻苯三酚法测定天葵子各部位和水层中的多糖对羟基自由基(·OH)和超氧自由基(O-2·)的清除能力,研究天葵子的抗氧化活性,并以此为导向分离主要活性成分。结果:天葵子抗氧化活性顺序为TK-D>TK-S>TK-Z>TK-Y,其中TK-D对超氧自由基的清除率与浓度呈正相关(Y=54.94X+27.15,R2=0.998 3),IC50为0.42 g·L-1;从正丁醇中分离得到5个化合物,分别鉴定为紫草氰苷(1),东方唐松草苷(2),格列风内酯(3),β-谷甾醇(4),胡萝卜苷(5)。结论:首次证实了天葵子提取物中水层和正丁醇层是其抗氧化活性部位,其中水层中的抗氧化活性物质为多糖,化合物紫草氰苷,东方唐松草苷,格列风内酯是天葵子的主要化学成分。
Objective: To study the antioxidant activity of different fractions and chemical constituents from Semiaquilegiae Radix in vitro. Method: Different extraction fractions from Semiaquilegiae Radix were ethyl acetate extraction( TK-Y),n-butanol extraction( TK-Z),water extraction( TK-S) and polysaccharide( TKD). Under the chemical simulation conditions in vitro,the antioxidant activities of different extraction fractions and polysaccharide were determined by ·OH,O-2· scavenging experiments. Furthermore,the chemical compounds were isolated from bio-active fraction. Result: The antioxidant activities of different extraction fractions from strong to weak were as follows: TK-D 〉 TK-S 〉 TK-Z 〉 TK-Y. Scavenging activity of TK-D for O-2· and·OH were positively correlated( Y = 54. 94 X + 27. 15,R2= 0. 998 3),IC50= 0. 42 g·L-1. Five major components were isolated and identified from TK-Z as lithtospermoside( 1),thalictricoside( 2),griffonilide( 3),β-sitosterol( 4),daucosterol( 5). Conclusion: The polysaccharide,n-butanol extraction and water extraction are active fractions and it identified the active fractions of Semiaquilegiae Radix for the first time. Lithtospermoside,thalictricoside and griffonilide were found as main contribution.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第15期116-119,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金青年基金项目(21102025)
贵州省科技厅中药攻关项目(黔科合ZY[2011]3006号)
贵阳医学院青年基金项目(2012017)