潜阳育阴颗粒对正常麻醉犬肾脏微循环的影响及其机制

Effects and Mechanism of Qianyang Yuyin Granules on Renal Microcirculation in Normal Dogs

目的：观察潜阳育阴颗粒（Qianyang Yuyin granules,QYG）对正常麻醉犬肾脏微循环影响,并初步研究其作用机制。方法：应用Power Lab系统记录十二指肠给予潜阳育阴颗粒前和给药后15~180 min不同时段比格犬肾脏微循环变化;犬分为正常组,QYG高、中、低剂量组（12.30,6.15,3.08 g·kg-1）,卡托普利组（30 mg·kg-1）和珍菊降压片组（0.14 g·kg-1）,于给药前及给药后15,30,60,90,120,180 min通过股动脉取血,ELISA法检测血清中血管紧张素Ⅱ（AngⅡ）,内皮型一氧化氮合酶（e NOS）含量,化学法检测一氧化氮（NO）含量;给药3 h后取肾脏组织,ELISA法检测肾脏AngⅡ含量。结果：潜阳育阴颗粒12.30 g·kg-1剂量组给药后60~180 min正常麻醉犬的肾动脉微循环血流量明显上升（P〈0.05）,给药180 min后肾脏组织AngⅡ含量明显下降（P〈0.05）,给药90,120,180 min后,血清中AngⅡ明显下降（P〈0.05）,e NOS和NO含量明显上升（P〈0.05）;潜阳育阴颗粒6.15 g·kg-1剂量组给药后90~120 min内正常麻醉犬的肾动脉微循环血流量明显上升（P〈0.05）,给药90,120 min后,血清中AngⅡ明显下降（P〈0.05）,e NOS和NO含量明显上升（P〈0.05）;潜阳育阴颗粒低剂量组给药后180min内正常麻醉犬各项指标变化不明显。结论：潜阳育阴颗粒可改善肾脏微循环,其作用机制可能与降低循环及肾脏组织AngⅡ的含量,增加循环中e NOS及NO含量有关。

Objective： To study the effects of Qianyang Yuyin granules（ QYG） on renal microcirculation in normal dogs and to investigate the mechanism. Method： The renal microcirculation was determined by Power Lab system before giving QYG（ 3. 08,6. 15,12. 30 g·kg-1） and after 15,30,60,90,120,180 min respectively. Result： Compared with the normal group,QYG（ 12. 30 g·kg-1） could increase the renal microcirculation（ P 〈 0. 05,60-180 min）,the contents of nitric oxide synthase（ e NOS）（ P 〈 0. 05） and nitric oxide（ NO） in the serum（ P 〈 0. 05,90-180 min） and reduce the contents of angiotensinⅡ（ AngⅡ） in the serum（ P 〈 0. 05,90-180 min） and kidney（ P 〈 0. 05,180 min）; QYG（ 6. 15 g·kg-1） could increase the renal microcirculation（ P 〈 0. 05,90-120 min）,the contents of e NOS and NO in the serum（ P 〈 0. 05,90-120 min） and reduce the contents of Ang Ⅱ in the serum（ P 〈 0. 05,90-120 min）. The effects of QYG（ 3. 08g·kg-1） were not obvious. Conclusion： QYG could improve renal microcirculation,its mechanism may be associated with the decreasing circulation and kidney tissue AngⅡ content,increasing the circulation of e NOS and NO contents.