红芸豆植物凝集素终止小鼠中晚期妊娠中的相关生殖激素变化研究

Changes of sexual hormones in mid-late pregnant mice after administration of red kidney beans lectin

目的:探讨红芸豆植物凝集素(PHA)在终止小鼠中晚期妊娠时对孕鼠相关神经内分泌激素水平的影响。方法:将妊娠14 d的孕鼠随机分为PHA组和溶剂对照(PBS)组,每组各40只。采用非手术法经阴道向每只孕鼠子宫注射5 mg的红芸豆PHA或等量的PBS,并于注射后2 h、5 h、8 h及发生流产时采血,取子宫胎盘(每个时间点每组处死10只动物),采用电化学发光法对孕鼠血清中的孕酮(P)、雌二醇(E2)、睾酮(T)、催乳素(PRL)、黄体生成素(LH)、卵泡刺激素(FSH)进行检测,比较妊娠结局;用派诺宁-甲基绿染色法观察子宫、胎盘石蜡切片。结果:注射PHA组孕鼠血清中E2、FSH、LH和T水平与注射PBS组无统计学差异;而P和PRL水平在注射红芸豆PHA后2 h时明显高于PBS组,流产时明显低于PBS组;PHA组胎盘出现明显细胞凋亡。结论:红芸豆PHA终止小鼠中晚期妊娠并不改变孕鼠体内的E2、FSH、LH和T水平,但对P和PRL有较大影响,提示红芸豆PHA通过作用于子宫局部而导致流产,不影响中枢神经内分泌系统。

Objective： To explore the changes of serum sexual hormones from pregnant mice with temination of＇middle and late pregnancy by administering red kidney bean phytohemagglutinin or lectin （PHA or RKBL）. Methods： Fourteen-day pregnant （mid-late period in pregnancy） mice were injected 5 mg PHA to one side of uterine by nonsurgical method trough vaginal. PBS was used in control group. Blood sample, uterine and placnta were collected from ten mice at each time of 2 h, 5 h, 8 h after PHA or PBS administration per group and sexual hormones above-mentioned were tested by electrochemical luminescence. Sexual hormones and pregnancy outcome between the two groups were compared. Paraffin section of uterine and placenta dyed by Pyronine-methyl green was observed. Results： There were no difference for luteinizing hormone （LH）, follicle-stimulating hormone （FSH）, estradiol （E2） and testosterone （T） between PHA group and PBS group. However, there were significantly differences on progesterone （P） and prolactin （PRL） between PHA group and PBS group. It showed that the P and PRL levels significantly increased compared with control group at 2 h and deceased significantly at abortion time compared with control group. An obvious apoptosis in placenta appeared in PHA group. Conclusion： Termination of mid-late pregnancy in mice by red kidney beans PHA did not change the E2, FSH, LH and T levels. But it changes the P and PRL levels in process of termination pregnancy. Red kidney beans PHA played the role in local uterine area and had no influences on hypothalamus-pituitary neuro-endocrine system.