摘要
目的研究FOXM1在乳腺癌组织中的表达与患者临床病理学特征的关系及其对乳腺癌细胞增殖能力的影响。方法在组织水平,用免疫组织化学法检测147例乳腺癌组织中FOXM1和Ki-67的表达,用卡方检验分析FOXM1的表达与临床病理学特征及Ki-67表达之间的关系。在细胞水平,对乳腺癌MCF-7细胞进行血清饥饿72 h后用Western blot法检测乳腺癌细胞周期。最后干扰MCF-7细胞中FOXM1的表达,分析细胞的增殖能力。结果FOXM1在乳腺癌组织中高表达,FOXM1与Ki-67的表达趋势一致。乳腺癌组织中FOXM1的表达与Her-2(P<0.05)、淋巴结转移(P<0.05)和Ki-67(P<0.05)相关。MCF-7细胞血清饥饿释放后增殖能力增强(P<0.05);敲低FOXM1表达水平后,MCF-7细胞的增殖能力减弱(P<0.05)。结论 FOXM1表达异常可能与乳腺癌的发生与进展关系密切;其可作为判断乳腺癌预后的指标和潜在的治疗靶点。
Objective To study the expression of FOXM1 in human breast cancer, analyze the relationship between FOXM1 and clinical pathological features and explore the role of FOXM1 in cell proliferation. Methods FOXM1 expression in breast cancer tissues was detected by immunohistoehemistry （IHC） on 147 parffin-embed- ded slices. X2 test was used to analyze the relationship of FOXM1, clinical parameters, as well as Ki-67. What＇s more, Western Blot was used to analyze the role of FOXM1 in cell cycle process during serum starvation and refeeding. At last, the expression level of FOXM1 was knocked down to analyze the alteration of MCF-7 cell vi- tality. Results FOXM1 was highly expressed in breast cancer. The expression of FOXM1 was consistent with the level of Ki-67. FOXMI expression in breast cancer was significantly correlated with Her-2, lymph node status, as well as Ki-67 （ P 〈 0.05 ）. With serum starvation for 72 hours and refeeding with the DMEM medium contain 5% fetal bovine serum in MCF-7 cells, the proliferation ability was enhanced （P 〈0. 05 ）. While interferenced with FOXM1, the proliferation ability was inhibited （ P 〈 0. 05 ）. Conclusions The abnormal expression of FOXM1 might be closely related to the initiation and progression of breast cancer. FOXM1 might be an indicator to predict the prognosis and serve as a potential target for therapy in breast cancer.
出处
《基础医学与临床》
CSCD
2015年第8期1100-1105,共6页
Basic and Clinical Medicine
