摘要
目的探讨微小RNA miR-181a对慢性粒细胞白血病(CML)K562细胞的作用。方法构建了miR-181a过表达的慢病毒载体,采用实时定量PCR方法检测CD235a和γ球蛋白的表达,评价K562细胞向红系分化的程度,检测CD61和CD41的表达,评价K562细胞向巨核系分化的程度,采用CCK实验检测细胞增殖情况。结果氯高铁血红素佛及波酯诱导后,miR-181a在K562细胞中过表达能够增强CD235a、γ球蛋白、CD61和CD41的表达水平(P<0.05);miR-181a过表达能够明显抑制K562细胞的增殖(P<0.05)。结论 miR-181a过表达促进K562细胞的分化并抑制其细胞的增殖。
Objective To study the effect of miR-181 a on chronic myelogenous leukemia(CML) K562 cells. Methods The miR-181a overexpression lentiviral vector was constructed, the differentiation degree of K562 cells to erythroid cells was evaluated by observing the expression of CD235a and gamma globulin, and the differentiation degree of K562 cells into megakaryocytic ceils was evaluated by observing the expression of CD61 and CD41 by real-time PCR. The proliferation of K562 cells was determined by CCK kit. Results The overexpression of miR-181a in K562 cells upregulated the expression levels of CD235a and gamma globulin and CD61 and CD41 after hemin and phorbol-12- myristate-13-acetate(TPA) induction; furthermore, the overexpression of miR-181a could significantly inhibit the proliferation of K562 cells(P〈0.05). Conclusion The overexpression of miR-18 la promotes the differentiation of K562 cells and inhibits the proliferation of K562 cells
出处
《解剖科学进展》
CAS
2015年第4期355-357,共3页
Progress of Anatomical Sciences
基金
黑龙江省自然科学基金项目(留学归国基金项目
No.LC2011C03)
黑龙江省中医管理局科研项目(No.ZHY12-Z057)