胎盘间充质干细胞对小鼠小肠缺血再灌注损伤的作用

The effect of placenta mesenchymal stem cells on small intestine ischemiareperfusion injury in mice

目的探讨胎盘间充质干细胞(pMSCs)对小鼠小肠缺血再灌注损伤的作用。方法组织块培养法提取pMSCs,流式细胞技术鉴定。24只小鼠随机分为假手术组(Sham组),缺血30min再灌注6h组(I/R组)和再灌注1h尾静脉输入5×10 6个pMSCs组。缺血再灌注6h后观察肠道情况,HE染色观察肠组织形态,ELISA检测血清中二胺氧化酶(DAO)、TNF-α、IL-6及IL-10含量。结果 pM SCs阳性表达CD29,CD44。与Sham组相比,I/R组肠道损伤明显,肠黏膜上皮细胞脱落多,而pM SCs组肠道损伤轻,细胞脱落少。I/R组和pM SCs组小鼠血清中DAO、TNF-α、IL-6及IL-10含量明显多于Sham组(P<0.05)。与I/R组相比,pM SCs组小鼠血清中DAO、TNF-α、IL-6含量减少(P<0.05),IL-10含量增多(P<0.05)。结论 pM SCs可减轻小鼠小肠缺血再灌注损伤,机制与抑制再灌注后炎症反应相关。

Objective To explore the effect of placenta mesenchymal stem cells（pMSCs） on intestinal ischemia-reperfusion injury in mice and and the possible mechanism. Methods The pMSCs were extracted by tissue culture method and identified by flow eytometry technology. 24 mice were divided into 3 groups：control group （Sham）, I/R group（isehemia for 30min then reperfusion for 6h and pMSCs group（injection of 5 × 10^5 pMSCs into tail vein after reperfusion for lh）. The intestinal morphology was detected by HE staining. The contents of diamine oxidase, tumor necrosis factor a, interleukin 6 and interleukin 10 in mice serum were detected by ELISA. Results The pMSCs expressed CD29 and CD44 positively. Compared with the sham group, the intestinal damages were more serious with much more intestinal mucosal epithelial cells falling off in FR group, but mush lighter in pMSCs group than in FR group. The contents of diamine oxidase, tumor necrosis factorot, interleukin 6 and interleukin 10 in mice serum were much higher in I/R group than in sham group, but much lower in pMSCs group than in FR group（P〈0.05） except for interleukin 10. Conclusion The alleviation of the damage of ischemiareperfusion small intestine by pMSCs might be related to the downregulation of the inflammatory response