唑来膦酸对骨质疏松性大鼠骨缺损修复的实验研究

Effect of zoledronic acid on repair of osteoporosis rats bone defect

目的探索唑来膦酸对骨质疏松性大鼠股骨干骺端骨缺损修复的影响。方法 30只3个月龄SPF级雌性SD大鼠,随机分为2组:1实验组,唑来膦酸注射组;2空白对照组,生理盐水注射组。将2组先去卵巢建立OVX(卵巢切除)模型,2组大鼠OVX模型饲养满3个月后,骨质疏松模型成功建立,随后再建立大鼠双侧股骨干骺端2.5 mm贯通性骨缺损模型。实验组于缺损手术后行尾静脉注射唑来膦酸1次(0.1 mg/kg),空白对照组给予等剂量的生理盐水尾静脉注射。分别于缺损术后6、12周进行X线观察,骨密度检测,HE骨组织切片观察,TRAP破骨细胞活性等检测。结果缺损术后6、12周,实验组和空白对照组对比发现:实验组的大鼠模型体重增加减慢,股骨骨密度增加变快;缺损区X线扫描较空白对照组有明显的新生骨生成,HE骨组织切片可见缺损区有分布密集的骨小梁和较多核深染的骨细胞。TRAP染色显示:实验组的破骨细胞活性降低,数量减少。结论唑来膦酸可较好地促进骨质疏松性大鼠股骨干骺端骨缺损的修复。

Objective To investigate the effect of zoledronic acid on the repair of defect of femoral epiphyseal end of osteoporosis rats. Methods Thirty 3-month-old SPF SD female rats were randomly divided into two groups： （1）the experimental group, zoledronic acid group; （2）the blank control group, saline group. AH rats were ovarieetomized and raised 3 months for osteoporosis model, and a 2.5 mm defect on the both femoral epiphyseal end was created. The experimental group was injected zoledronic acid （0.1 mg/kg） through tail vein, and blank control group was given the same amount of saline the same way. The X-ray observation, histopathological observation, and the TRAP osteoclast activity detection were completed respectively at 6 and 12 weeks after surgery. Results Six and 12 weeks after the operation, the experimental groups had a slower weight gain, and BMD increased faster; X-ray scan on the defect area showed that more bone tissue was generated compared with blank control group. In the experimental groups, a large number of trabeeular bone and＇more hyperehromatism-nuelear bone cells were observed in the pathological section of the defect area. TRAP showed that the activity of osteoclast was restrained and the number of the osteoclast declined in experimental groups. Conclusion The zoledronie acid can promote the repair of the defect in femoral epiphyseal end of osteoporosis rats.