摘要
目的:探讨mi R-101在肝细胞癌(HCC)细胞生物学行为的影响。方法:分别将mi R-101模拟物或mi RNA阴性对照序列转染Hep G2和SMMC-7721两种HCC细胞,以无处理自然生长的两种细胞为各自空白对照,观察mi R-101对两种细胞增殖、集落形成能力、凋亡及周期的影响,以及对侵袭和迁移能力的影响。结果:与各自的空白对照细胞比较,转染mi R-101模拟物后的Hep G2和SMMC-7721细胞的增值能力明显降低、细胞集落的形成明显减少、G0/G1期的细胞比例升高、细胞凋亡率明显增加、迁移与侵袭细胞数明显减少(均P<0.05);转染mi RNA阴性对照序列对两种细胞的以上指标无明显影响(均P>0.05)。结论:mi R-101可能在HCC细胞中起到了抑癌基因的作用,上调其表达能抑制HCC的恶性生物学行为。
Objective: To investigate the influence ofmiR-101 on the biological behavior ofhepatocellular carcinoma (HCC) cells. Methods: The HCC HepG2 and SMMC-7721 cells were transfected with miR-101 mimics or miRNA negative control sequences, using the two types of cells cultured without any treatment as corresponding blank control, and then the effect of miR-101 on proliferation, colony formation ability, apoptosis and cell cycle, as well as the migration and invasion ability of the two types of cells were observed. Results: Compared with corresponding blank control, in either HepG2 or SMMC-7721 cells after transfection with miR-101 minics, the proliferative ability was decreased, colony formation was reduced, G0/G1 phase cell ratio was increased, apoptosis rate was increased, and the ability of migration and invasion was reduced markedly (all P〈0.05); transfection of miRNA negative control sequences exerted no obvious effect on above parameters for the two types of cells (all P〉0.05). Conclusion: miR-101 possibly plays a role as cancer suppressor gene in HCC cells, and upregulating its expression may suppress the malignant activity of HCC.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2015年第7期963-969,共7页
China Journal of General Surgery
关键词
癌
肝细胞
微RNAS
肿瘤侵润
Carcinoma, Hepatocellular
MicroRNAs
Neoplasm Invasiveness