上调miR-101对肝细胞癌生物学行为的影响

Impact of upregulating miR-101 on biological behavior of hepatocellular carcinoma

目的:探讨mi R-101在肝细胞癌(HCC)细胞生物学行为的影响。方法:分别将mi R-101模拟物或mi RNA阴性对照序列转染Hep G2和SMMC-7721两种HCC细胞,以无处理自然生长的两种细胞为各自空白对照,观察mi R-101对两种细胞增殖、集落形成能力、凋亡及周期的影响,以及对侵袭和迁移能力的影响。结果:与各自的空白对照细胞比较,转染mi R-101模拟物后的Hep G2和SMMC-7721细胞的增值能力明显降低、细胞集落的形成明显减少、G0/G1期的细胞比例升高、细胞凋亡率明显增加、迁移与侵袭细胞数明显减少(均P<0.05);转染mi RNA阴性对照序列对两种细胞的以上指标无明显影响(均P>0.05)。结论:mi R-101可能在HCC细胞中起到了抑癌基因的作用,上调其表达能抑制HCC的恶性生物学行为。

Objective： To investigate the influence ofmiR-101 on the biological behavior ofhepatocellular carcinoma （HCC） cells. Methods： The HCC HepG2 and SMMC-7721 cells were transfected with miR-101 mimics or miRNA negative control sequences, using the two types of cells cultured without any treatment as corresponding blank control, and then the effect of miR-101 on proliferation, colony formation ability, apoptosis and cell cycle, as well as the migration and invasion ability of the two types of cells were observed. Results： Compared with corresponding blank control, in either HepG2 or SMMC-7721 cells after transfection with miR-101 minics, the proliferative ability was decreased, colony formation was reduced, G0/G1 phase cell ratio was increased, apoptosis rate was increased, and the ability of migration and invasion was reduced markedly （all P〈0.05）; transfection of miRNA negative control sequences exerted no obvious effect on above parameters for the two types of cells （all P〉0.05）. Conclusion： miR-101 possibly plays a role as cancer suppressor gene in HCC cells, and upregulating its expression may suppress the malignant activity of HCC.