水稻种质资源萌发期抗旱性综合鉴定

Identification of drought tolerance of rice germplasm during germination period

为完成水稻种质资源萌发期的抗旱性评价并筛选出抗旱性评价指标,利用PEG-6000高渗溶液模拟干旱条件,对33份水稻材料在胁迫条件下的发芽势、发芽率、最大胚根长、胚芽长、胚芽鞘长、根冠比、物质转运速率等11个萌发性状指标进行测定。结果表明,部分性状指标间的相关性达到显著水平,其中物质转运速率与根干重间的相关性最高,相关系数为0.83,根冠比与胚芽干重和胚芽长存在极显著负相关,相关系数分别为-0.51和-0.47;主成分分析将11个生长指标归类为4大因子,各因子中负荷量最大的指标为胚芽干重、发芽率、胚芽鞘长和根冠比,相关系数分别为0.4876、0.5266、0.6661、0.5988,可作为水稻抗旱性鉴定的主要指标;以各指标的隶属函数值进行模糊聚类分析,根据抗旱性强弱分为4大类,其中XS-13-12和XS-13-9为高度抗旱品种(系),XS-13-2、A218、XS-13-11等11份水稻材料为抗旱品种(系),稻花香、SN1204、XS-13-13、XS-13-3等14份水稻材料为中等抗旱品种(系),1043、SN9903、XS-13-4等6份水稻材料为干旱敏感品种(系)。

The study aims to assess drought of 30 rice cultivars during germination period,using PEG- 6000 hypertonic solution to simulate drought condition. Eleven traits germinating performance were investigated,such as germination force,germination values,root length,germ length,coleoptile length,ratio of root to shoot,biomass transport rate. The results showed significant correlations between biomass translocation rate and ratio of root to shoot（ 0. 83） and the ratio of root / shoot and germ dry weight（- 0. 51） and length（- 0. 47）. Principal component analysis showed that the 11 traits were classified as four major factors,and germ dry weight,germination rate,coleoptile length,and the ratio of root to shoot showed maximum load,with the correlation coefficients being 0.4876,0. 5266,0. 6661 and 0. 5988,respectively. These factors have implications in the identification of drought resistance in rice. clustering analysis divided rice drought resistance into four categories,with XS- 13- 12 and XS- 13- 9 being highly drought resistant,XS- 13- 2,A218 and XS- 13- 11 resistant,Daohuaxiang,SN1204,XS- 13- 13 and XS- 13- 3 moderate resistant,while 1043,SN9903 and XS- 13- 4 drought sensitive.