棒状简枝霉YX016产蒽醌发酵条件的优化研究

Optimization on fermentation conditions of Simplicillium obclavatum YX016 for the production of anthraquinones

目的:从竹黄中分离筛选到可以发酵产生蒽醌的一株棒状简枝霉YX016,为提高蒽醌产量,对液体发酵条件进行优化。方法:通过单因素实验选出合适的碳源、氮源、微量矿质元素和生长因子等,再以葡萄糖、尿素、KH2PO4、Mg SO4、H3BO3、生长因子VB1和初始p H进行7因素4水平的正交优化实验。结果:确定的最佳培养基配方为葡萄糖30 g/L、尿素0.5 g/L、KH2PO4 0.2 g/L、Mg SO4 0.05 g/L、H3BO3 0.08 g/L、VB1 21μg/100 mL,初始p H6.5,装液量为100 mL/250 mL,接种菌龄为5 d,发酵温度为27℃,摇床转速为150 r/min。在优化的发酵条件下,蒽醌的产量达到69.05μg/m L,比优化前提高了1.95倍。

Objective：A strain of producing anthraquinones,Simplicillium obclavatum YX016,was isolated from the fruiting body of Shiraia bambusicola.The liquid culture conditions for anthraquinones were optimized in order to enhance the production of anthraquinones.Methods：The effects of carbon source,nitrogen source,trace mineral elements,growth factors and rotatory speed on the production of anthraquinones was researched by means of single-factor test.Furthermore,the orthogonal test which contained seven factors and four levels was employed to evaluate the effects of glucose,urea,KH_2HPO_4,MgSO_4,H_3BO_3,V_（B1） and initial pH on the production of anthraquinones.Results：The optimal medium formulation was determined as follows：glucose 30 g/L,urea 0.5 g/L,K_2HPO_4 0.2 g/L,MgSO_4 0.05 g/L,H_3BO_3 0.08 g/L,V_（B1） 21 μg/100 mL,initial pH 6.5,medium volume 100 mL was in 250 mL flask,inoculant age of 5 d,incubation temperature 27 ℃,rotatory speed 150 r/min.Under the optimal conditions,the yield of anthraquinones could reach 69.05 μg/mL,which was 1.95 folds compared to that in the basic culture medium.