摘要
以神秘果果实为实验材料,以改良CTAB法提取并获得高质量的总果实RNA,经过RTPCR获得Miraculin基因,构建原核表达载体pQE30-MIR并进行原核表达。经过大量的实验,表明Miraculin蛋白可溶表达的最佳条件为:28℃,120 r/min,IPTG使用浓度为1.5 mmol/L,诱导表达5 h。
Hight quality total RNA was obtain from miracle fruits using CTAB improved method,and the miraculin gene was cloned through RT-PCR.The prokaryotic expression vector pQE30-MIR was constructed and studied on the conditions of soluble expression.Lots of experiments showed that the best conditions for soluble expression of miraculin was：28 ℃,120 r/rmin,IPTG 1.5 mrmol/L,induced for 5 hours.
出处
《食品科技》
CAS
北大核心
2015年第7期36-40,共5页
Food Science and Technology
基金
广东高校特色调味品工程技术开发中心建设项目(GCZX-B1103)
关键词
神秘果素
变味蛋白
可溶表达
miraculin
taste-modifying protein
soluble expression
