金属离子对巨大口蘑褐变相关酶活力的影响

Effect of the brown enzyme activity of Tricholoma giganteum under different metal ions treatment

巨大口蘑是一种珍稀食用菌,具有抗褐变特性,了解其褐变相关酶的特性,对探明其抗褐变机理具有重要意义。采用不同金属离子（Zn^2＋、Mg^2＋、Cd^2＋、Cu^2＋、Fe^2＋、Fe^3＋、Mn^2＋）的梯度溶液浸泡法处理蘑菇切片,并测定了不同金属离子对其褐变相关酶活力影响。结果表明：Cu^2＋、Zn^2＋、Mg^2＋对其多酚氧化酶（PPO）具有显著低促高抑作用,Fe^3＋为激活作用,Fe^2＋和Cd^2＋起抑制效果。0.4-2 mmol/L的Zn^2＋、Mg^2＋和Cd^2＋对其酪氨酸酶（TYE）有显著激活作用,Cu^2＋具有低促高抑作用;Fe^3＋、Fe^2＋和Mn^2＋对TYE活力影响不显著。Cd^2＋、Cu^2＋、Fe^3＋、Mn^2＋和Zn^2＋对其苯丙氨酸解氨酶（PAL）有显著激活效果,Fe^2＋对PAL有抑制作用;Mg^2＋对PAL无显著影响。

In order to explore the mechanism of Trichoioma giganteum anti-browning,the gradient solution immersion method with different metal ions（Zn^2＋,Mg^2＋,Cd^2＋,Cu^2＋,Fe^2＋,Fe^3＋,Mn^2＋） was adopted to process mushroom slices,and the influence of different metal ions on the browning related enzyme activity was determinated in this paper.The results showed that Cu^2＋,Zn^2＋,Mg^2＋ had significant stimulation on the poiyphenoi oxidase（PPO） activity at low concentration and had inhibition at high concentration.Fe^3＋ could active PPO activity.Fe^2＋ and Cd^2＋ had inhibitory effect.0.4-2 mmol/L Zn^2＋,Mg^2＋ and Cd^2＋ had significant activation on the tyrosinase（TYE） activity.Cu^2＋ had stimulation at low concentration and had inhibition at high concentration.The influence of Fe^3＋,Fe^2＋ and Mn^2＋ on the TYE activity was not significant.Cd^2＋,Cu^2＋,Fe^3＋,Mn^2＋ and Zn^2＋ had significant activation on the phenylalanine ammonialyase（PAL） activity.Fe^2＋ had inhibition on PAL;Mg^2＋ had no significant influence on PAL activity.