响应面法优化复合酶法提取猴头菇多糖的工艺研究

Response surface optimization of complex-enzyme method for extraction of polysaccharides from Hericium

采用复合酶法对猴头菇多糖进行提取,以猴头菇多糖得率和纯度的综合指标为评价指标,研究了酶添加量、酶解温度、酶解时间、酶解p H 4个因素对提取效果的影响。在单因素试验的基础上,通过Box-Behnken响应面法优化猴头菇多糖的提取工艺条件。建立了多糖综合指标Y与酶添加量A、酶解温度B、酶解时间C、酶解p H D之间的回归方程:Y=24.91+0.011A-0.38B-0.100C-0.22D-0.27AB+0.024AC+3.250E-003AD-0.61BC+6.250E-003BD+1.500E-003CD-0.79A2-0.79B2-1.19C2-0.75D2。最终确定最佳酶解条件为酶添加量0.51%,酶解温度48.71℃,酶解时间100.50 min,酶解p H4.43。在此条件下多糖的得率为19.22%,纯度为38.39%,综合指标为24.97%。

Polysaccharide was extracted from Hericium by complex-enzyme method.The influences of enzyme dosage,extraction temperature,extraction time and pH were investigated on the extraction rate and purity.On the basic of single factor test,the best possible extraction parameters were obtained with the response surface methodology,at a four variable,three-level experiment Box-Behnken design（BBD）.The regression equation was Y=24.91＋0.011A-0.38B-0.100C-0.22D-0.27AB＋0.024AC＋3.250E-003AD-0.61BC＋6.250E-003BD＋1.500E-003CD-0.79A^2-0.79B^2-1.19C^2-0.75D-2.The optimum extraction parameters were as follows：the enzyme dosage was 0.51% ,the extraction temperature was 48.71 ℃,the extraction time was 100.50 min and the pH was 4.43.The extraction rate was 19.22% ,the purity was 38.39% and the complex indicator was 24.97% under these conditions.