HPLC法测定云南青牛胆清除DPPH自由基活性能力

DPPH radical scavenging activity of the extract from Tinospora sagittata var.yunnanensis

采用HPLC法研究了不同溶剂对云南青牛胆提取物清除1,1-二苯基-2-三硝基苯肼（DPPH）自由基测定的方法。实验结果表明,云南青牛胆提取物具有良好的自由基清除能力,其顺序为：乙醇〉乙酸乙酯〉正丁醇〉丙酮。其中,乙醇提取物（IC50为0.69 mg/m L）的清除能力最大,除丙酮提取物（IC50为1.93 mg/m L）外,其他提取物的自由基清除能力均大于香草酸（IC50为1.82 mg/m L）的清除能力;相关分析结果表明,各提取物在一定浓度范围内与自由基清除率呈现良好的线性关系,DPPH自由基浓度c在0.1-1.0 mg/m L的浓度范围内与峰面积A呈线性相关,A=11146c-1083.3,r=0.9991。

DPPH（1,1-pheny-2-picrylhydrazyl） radical scavenging activities of the extracts of Tinospora sagittata var.yunnanensis in different solvent were analyzed by HPLC methods.The experimental results showd that the extracts have a good ability of DPPH radical scavenging activities,and the order is ethanolethyl acetaten-buohacetone.Among them,the ethanol extract（IC50 was 0.69 mg/mL） removal capacity is the largest,in addition to the acetone extracts（IC50 was 1.93 mg/mL）,the DPPH radical scavenging capacity of other extract is greater than the vanillic acid（IC50 was 1.82 mg/mL）.There are good linear relationships between the extracts in the certain concentration range and scavenging free radicals.Similarly,the mass concentration of DPPH radical in the range of 0.1 -1.0 mg/mL and the peak area also has a good linear relationship,the regression equation and the correlation coefficient is A=11146C-1083.3and 0.9991,respectively.