腺病毒介导脂联素对干燥综合征小鼠颌下腺炎症的影响

Effects of adenovirus mediated AdipoQ on mice submandibular gland in Sjogren＇s syndrome

目的观察脂联素在ss小鼠颌下腺的表达；通过体内转染重组基因，探讨脂联素在ss小鼠炎症中的作用。方法选取30只非肥胖糖尿病（NOD）小鼠作自发性ss小鼠模型。分别转染脂联素基因重组腺病毒，空病毒载体和生理盐水（对照组），比较3组小鼠颌下腺腺体、病理学改变，TNF-α、脂联素水平。用实时荧光定量（RT）-PCR检测小鼠腺体脂联素表达。结果通过3组小鼠对比发现，对照组小鼠腺体破坏，淋巴细胞浸润，腺泡细胞萎缩变性，rADV一腺病毒组无淋巴细胞浸润；与对照组相比，rADV-腺病毒组TNF-α、颌下腺脂联素mRNA表达[（248±30）和（162±73）ng／m1]均较对照组显著降低（P〈0．05）；对照组间差异无统计学意义（P〉0．05）。结论局部转染脂联素可明显改善小鼠腺体形态学、降低TNF-α水平，抑制小鼠涎腺炎症反应，降低脂联素表达，脂联素可能为ss小鼠保护基因。

Objective To observe the expression of AdipoQ in Sjogren＇s syndrome （SS） mice and its role in inflammation was investigated by recombinant gene transfection study in vivo. Methods As spon- taneous SS mice model, a total of 30 NOD mice were divided into 3 groups randomly： recombinant adenovirus （rADV-AdipoQ） group, normal saline control and simple adenoviruses （control group）. The submandibular gland morphology, histopathological grading and the level of serum tumor necrosis factor-or （TNF-α, AdipoQ was compared between the three groups. The expression of AdipoQ on mice submandibular gland was assessed by means of semi-quantitative reverse transcriptase polymerase chain reaction. Results The submandibular glands of the mice of the control group were destructed, with focal lymphocytic infiltration and acinus atrophy. Compared with control model groups, the serum TNF-α and salivary gland AdipoQ expression was sig^cantly down-regulated in the rADV-AdipoQ group [（248±30） vs （162±73） ng/ml] （P〈0.05）. Conclusion AdipoQ gene transfected SS mice can significantly improve the morphological features of tissues and decrease the concentration of TNF-α in serum, in addition, it can effectively inhibit inflammation, decrease the degree of protein and AdipoQgene expression. So the AdipoQ may be the protective gene in SS mice.