Toll样受体9 2848G／A基因多态性与显微镜下多血管炎的关系

The correlation between toll-like receptor 9 gene 2848G/A polymorphism and primary anti-neutrophil cytoplasmic antibodies associated small vasculitis

目的研究TLR9 2848G／A基因多态性与ANCA相关性小血管炎（AAV）的关系。方法采用病例一对照研究，筛选135例AAV（病例组）和140名健康对照人群（对照组）作为研究对象，对其经行血清生化指标的检测，并使用限制性片段长度多态性聚合酶链反应（PCR-RFLP）方法对TLR9 2848G／A位点进行分析。采用t检验、方差分析、秩和检验、X^2检验进行统计分析。结果①135例AAV患者TLR9 2848G／A存在3种基因型，AA、GG、GA3种基因型频率分别为14．1％、38．5％、47．7％。②ANCA相关性小血管炎患者组AA基因型IgM水平高于GG＋GA基因型（居4．561，P〈0．05）。③病理分析显示：3种基因型的患者肾脏病理活动性评分和慢性评分差异无统计学意义（F=2．115，0．760，P〉0．05）。结论中国广西壮族自治区人群中，TLR9 2848G／A基因多态性可能与IgM升高具有相关性，可能与AAV的遗传易感性不相关。

Objective To investigate the correlation between toll-like receptor 9 （TLR9） gene 2848G/A polymDrphism and primary antineutrophil cytoplasmic antibodies （ANCA） associated small vasculitis （AAV）. Methods A case-control study was performed among 135 patients diagnosed,with AAV and 140 disease-free control and we test the serum biochemical parameter. Polymorphism was analyzed by polymerase chain restricted fragments length polymorphism. As for statistic method, according to the character of data, we performed t-test, chi-square test, Spearman grade related analysis and one-way ANOVA. Results ① The frequencies of AA, GG, GA genotype of TLR9 2848 in AAV patients were 14.07%, 38.52%, and 47.71%, respectively; ② Significant increase in IgM was observed in AA genotype than GG＋GA genotype in AAV patients （F=4.561, P〈0.05）. ③ There was no significant difference between AI and CI in AA, GA and GG genotype in AAV patients （F=2.115, 0.760, P〉0.05）. Conclusion AA, GA and GG genotypes are detected in TLR9 2848G/A in patients with AAV in Guangxi, without significant correlation with susceptibility to primary AAV in Guangxi.