HPV基因分型对宫颈非典型鳞状上皮细胞的临床分流价值

Clinical value of HPV genotyping in screening patients with atypical squamous cells of undetermined significance(ASC-US)

目的了解人乳头瘤病毒（HPV）基因分型检测对不能明确意义的非典型鳞状上皮细胞（ASC-US）的临床分流价值。方法对115例TCT（thin-prep cytology test）检查结果为ASC-US的病例进行HPV基因扩增分型检测以及阴道镜和组织病理学检查。结果 115例ASC-US患者中组织病理学诊断为炎症的有57例（49.6%）,子宫颈上皮内瘤变Ⅰ级（CINⅠ）22例（19.1%）,CINⅡ~Ⅲ级27例（23.5%）,浸润性鳞状细胞癌9例（7.8%）。其中高危型HPV阳性者52例,子宫颈高级别鳞状上皮内病变在高危型HPV阳性组和阴性组的发生率分别为50.0%和15.9%,差异有统计学意义（P〈0.01）。结论 HPV基因分型检测对ASC-US病例有重要的临床分流价值。

Objective To investigate the clinical value of genotyping of human papilloma virus（HPV） in screening the patients with atypical squamous cells of undetermined significance（ASC-US）. Methods 115 cases with ASC-US diagnosed by thin-prep cytology test（TCT） underwent gene amplification and HPV genotyping as well as colposcopic examination and histopathological examination. Results Among 115 cases with ASC-US, 57 cases（49.6%） were histopathologically diagnosed with inflammation, 22 cases（19.1%） with CIN I, 27 cases（23.5%） with CIN II and III, and 9 cases（7.8%） with invasive squamous cell carcinoma. Among the 52 cases with high-risk HPV positive, the incidence of cervical intraepithelial neoplasia in high-risk HPV positive group and negative group was 50% and 15.9%, respectively, and the difference was statistically significant（P〈0.01）. Conclusion HPV genotyping can be of important clinical value in screening ASC-US cases.