毛蕊花糖苷对内毒素诱导的急性肺损伤的保护作用

Acteoside attenuates acute lung injury induced by lipopolysaccharide in mice

目的:探讨毛蕊花糖苷对内毒素(lipopolysaccharide,LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用。方法:于小鼠腹腔注射LPS(8 mg/kg)复制ALI动物模型。将小鼠随机分为空白组对照组、LPS组、地塞米松组(2 mg/kg)、毛蕊花糖苷组(20 mg/kg、40 mg/kg)。观察各组肺组织病理学改变,测量肺湿/干重比,支气管肺泡灌洗液中性粒细胞百分比、超氧化歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、前列素E2(PGE2)含量及肺组织中环氧酶-2(COX-2)5-脂氧合酶(5-LOX)蛋白表达。结果:毛蕊花糖苷组(20 mg/kg、40 mg/kg)可有效减轻LPS所致肺组织病理学变化,能降低肺泡灌洗液中炎症细胞的数目、MDA、IL-6、TNF-α、PGE2,增加肺泡灌洗液中SOD;著降低肺湿/干重比重,减低COX-2与5-LOX蛋白水平。结论:毛蕊花糖苷20 mg/kg、40 mg/kg可减轻LPS所致急性肺组织损伤,对LPS诱导的急性肺损伤有保护作用。

Objective： To determine the protective effect of acteoside on acute lung injury induced by lipopolysaccharlde （LPS）. Methods： The BABL/C mice were randomly allocated into control, LPS, dexamethasone （2mg/kg）, acteoside （20 mg/kg）, and acteoside （40 mg/kg）. The bronchoalveolar lavage fluid （BALF） samples were analyzed for the concentration of numbers of neutrophilic, SOD, MDA, prostaglandin E2 （PGE2）, interleukin-6 （IL-6） and tumor necrosis factor alpha （TNF-ct）, and The lung samples were taken for histologlc evaluation and for determination of wet-to-dry（W/D） lung weight, The protein levels of cyclooxygenase-2 （COX-2） and 5-1ipoxygenase were detected by Western blot analysis. Results： Pretreatment with acteoside prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight/dry weight ratio, in total leukocyte number and neutrophil percent in the BALF, and in PGE2 of lung in dose-dependent man- ners, and in IL-6, the tumor neurosis factor-or （TNF-ct） in the BALF and COX-2 and 5-LOX proteins in lung. These results showed that an- ti-inflammatory effects of acteoside against the LPS-induced ALI.