3种排龈线不同时间浸提液的细胞毒性研究

Cytotoxic effects of the extractions of three gingival retraction cords with different extraction time

目的:观察3种排龈线在不同浸提时间下的细胞毒性。方法:用DMEM细胞培养液制备肾上腺素排龈线、硫酸铝排龈线、不含药排龈线分别浸提5、10、15、30 min的浸提液,用于培养人牙龈成纤维细胞(HGFs)。采用MTT比色法观察HGFs增殖活性;Annexin/PI双染法检测HGFs凋亡。结果:3种排龈线的不同浸提时间浸提液都可抑制HGFs增殖,诱导HGFs凋亡,随着浸提时间延长(15、30 min),HGFs的早期凋亡率逐渐增加,显著高于空白对照组(P<0.05)。结论:3种排龈线具有一定程度时间依赖性细胞毒性。

Objective： To evaluate the toxic effect of 3 different gingival retraction cords. Methods： DMEM extraction of DL-adren- aline HC1, aluminium sulphate and non-drug retraction cords with the extraction time of 5, 10, 15 and 30 min were respectively pre- pared and were used to culture human gingival fibroblasts （HGFs） in vitro respectively. Cell proliferation was tested by MTF assay. Cell apoptosis was examined by Annexin/PI method. Results： The 3 gingival retraction cord extractions inhibited the roliferation, pro- moted the apoptosis of HGFs（P 〈 0.05 ）, the effects were related to the extraction time. Conclusion： The 3 retraction cords have time-dependant cytotoxity.