抗纤灵方对5/6肾切除小鼠肾衰模型肾纤维化作用及机制研究

Kangxianling Decoction to 5/6 Nephrectomy Model of Mice Renal Fibrosis Mechanism

目的研究抗纤灵方对5/6肾切除小鼠肾纤维化的作用及机制研究。方法将60只C57小鼠,随机分为假手术组10只和手术组50只,手术组行5/6肾切除术。术后2周,手术组随机分为模型组、抗纤灵低、中、高剂量组及雷帕霉素阳性对照组,各组10只。各组于术后2周分别给予生理盐水、雷帕霉素及相应剂量的抗纤灵灌胃。治疗12周后处死小鼠,取肾组织检测肾脏病理(HE,MASSON染色);应用免疫荧光法检测肾组织平滑肌肌动蛋白(a-SMA)表达,采用PCR法检测a-SMA及纤维粘连蛋白(FN)表达。结果与假手术组比较,模型组病理损伤程度显著增加,a-SMA及FN表达明显(P<0.01);与模型组比较各治疗组病理损伤减轻,a-SMA及FN表达减少(P<0.05),且呈剂量依赖关系;高剂量组与雷帕霉素组比较差异无显著性(P>0.05)。结论抗纤灵能改善肾脏纤维化,延缓肾脏病进展,其机理可能与下调肾脏a-SMA及FN表达相关。

Objective To research Kangxianling Decoction on 5 /6 nephrectomy in mice renal fibrosis mechanism.Methods 60C57 mice were randomly divided into sham operation group of 10 and group of 50,the surgery group underwent 5 /6 nephrectomy.After two weeks,the surgery group were randomly divided into model group,Kangxianling low,medium and high dose groups and rapamycin positive control group,10 in each group.Each group were given normal saline after two weeks,and the corresponding doses of rapamycin Kangxianling gavage.Mice were sacrificed after 12 weeks of treatment,the renal tissue to detect renal pathology（ HE,MASSON）; immunofluorescence assay in renal tissue smooth muscle actin（ a- SMA） expression was detected by PCR method a- SMA and fibronectin（ FN） expression.Results Compared with the sham group,model group pathological degree of injury increased significant,a- SMA and FN expression was significantly（ P〈0.01）; compared with model group in each treatment group pathological damage mitigation,a- SMA and FN expression was reduced（ P〈0.05） in a dose- dependent manner; high dose group and the rapamycin group had no significant difference（ P〉0.05）.Conclusion Kangxianling can improve renal fibrosis,slow progression of renal disease,the mechanism may be related to reduced renal expression of a- SMA and FN- related.