正品青叶胆遗传多样性的ISSR分析

Analysis of genetic diversity in certified Swertia mileensis by ISSR

目的对青叶胆（Swertia mileensis）种质资源的遗传多样性进行研究。方法采用ISSR分子标记技术分析青叶胆的遗传多样性。利用NTSYS软件分析遗传距离及相似系数,UPGMA方法聚类,构建亲缘关系系统图。结果从30个ISSR引物中筛选出8个用于多态性分析,8条引物共检测到82个位点,其中79个位点具有多态性,占96.34%。遗传相似系数变化范围0.3537~0.9756。结论聚类结果显示,来源于同一地区不同居群的青叶胆亲缘关系较近,并与其近缘种具有显著差异。该实验从分子水平为青叶胆及其近缘种药用植物的鉴定和开发提供了遗传基础。

Objective To investigate the genetic diversity for germplasm resources of Swertia mileensis.Methods The genetic diversity of Swertia mileensis was analyzed by ISSR molecular markers.To make up the systematic diagram of genetic relationship by NTSYS software,cluster by UPGMA method,and establish the dendrogram.Results Eight ISSR primers were selected respectively from 30 ISSR primers.Eight primers produced 82 sites,among which 79 were polymorphic sites.The average percentage of polymorphic bands was 96.34%.Genetic similarity coefficient changed from 0.3537 to 0.9756.Conclusion By cluster analysis,it shows that different populations of the medicinal plants in Swertia mileensis from the same region are in the same group and demonstrates there are significant differences between its counterfeit species in the tested materials.This study will provide the foundation for identification and development of medicinal plants in Swertia mileensis.