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基于基因克隆的远志鲨烯环氧酶(SE)蛋白结构及进化分析

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摘要 目的基于基因克隆对远志鲨烯环氧酶(SE)进行蛋白结构及进化分析。方法克隆远志鲨烯环氧酶基因(SE)DNA片段,将已克隆SE基因翻译成氨基酸序列,进行蛋白结构及进化分析。结果已克隆远志鲨烯环氧酶基因DNA片段长517bp,具有170个氨基酸,有三个特征位点:Protein kinase C phosphorylation site、N-myristoylation site与Tyrosine kinase phosphorylation site,远志SE基因与Medicago sativa和Medicago truncatula的SE基因亲缘关系较为接近。结论已克隆远志SE基因DNA片段能够推测远志鲨烯环氧酶部分特征位点,可以进行远志SE蛋白进化分析。
出处 《时珍国医国药》 CAS CSCD 北大核心 2015年第7期1791-1792,共2页 Lishizhen Medicine and Materia Medica Research
基金 国家自然科学基金(No.31270381) 成都中医药大学优秀博士研究生科研创新基金一等资助项目(No.070104)
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  • 1赵云生,万德光,陈新,李占林,田洪岭.五环三萜皂苷生物合成与调控的研究进展[J].中草药,2009,40(2):327-330. 被引量:24
  • 2Suzuki H. , Achnine L. , Xu R. , et al. A genomics approach to the early stages of triterpene saponin biasynthesis in Medicago truncatula [ J ]. Plant J. 2002,32(6) :1033.
  • 3He F. ,Zhu Y. , He M. et al. Molecular cloning and characterization of the gene encodingsqualene epoxidase in Panax notoginseng [ J ]. DNA Seq. 2008:19 (3) :270.
  • 4Ono T, Nakazono K, Kosaka H. Purification and partial characterization of squalene epoxidase from rat liver microsomes [ J ]. Biochim Biophys Acta 1982,709( 1 ) :84.
  • 5Abe I, Seki T, Umehara K, et al. Green tea polyphenols: novel and po- tent iuhibitors of squalene epoxidase [ J ]. Biochem Biophys Res Com- muu, 2000,268 ( 3 ) :767.
  • 6Stefanie L. Butland, Monica L. Chow and Brian E. Ellis. A diverse family of phenylalanine ammonia - lyase genes expressed in pine trees and cell culture[J]. Plant molecular Biology,1998,37: 15.
  • 7萨姆布鲁克J,弗里奇EF,曼尼阿蒂斯T.分子克隆实验指南,第3版[M].北京:科学出版社,2002:100.

二级参考文献21

  • 1陈莉,吴耀生.三萜皂苷生物合成途径及相关酶[J].国外医药(植物药分册),2004,19(4):156-161. 被引量:15
  • 2邢朝斌,王一曼,陈正恒,沈海龙.三萜皂苷的生物合成[J].生命的化学,2005,25(5):420-422. 被引量:17
  • 3程晓华,熊玉卿.五环三萜皂苷的药理作用研究进展[J].中草药,2007,38(5):792-795. 被引量:59
  • 4Bach T J. Some new aspects of isoprenoid biosynthesis in plants: a review [J]. Lipids, 1995, 30: 191-202.
  • 5Mcgarvey D, Cro-re-au R. Terpenoid metabolism [J]. Plant Cell, 1995, 7: 1015-1026.
  • 6Lichtenthaler H K, Schwender J, Disch A, et al. Biosynthesis of isoprenoids in higher plant chloroplasts proceeds via a mevalonate-independent pathway [J]. FEBS Lett, 1997, 400 (3) :271-274.
  • 7Kajiwara S, Fraser P D, Kondo K, et al. Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli [J]. Biochem J, 1997, 324(2): 421-426.
  • 8Sun Z R, Cunningham F X, Gantt E. Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte[J]. Proc Nat Acad Sci USA, 1998, 95(19) : 11482-11488.
  • 9Concepcion R. Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli [J]. FEBS Lett, 2000, 473(3): 328-332.
  • 10Choi D, Ward B L, Bostock R M. Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor araehidonic acid [J]. Plant Cell, 1992, 4(10): 1333-1344.

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