HPLC法测定复方鲜竹沥液中对羟基苯甲酸乙酯的含量

Determination of P-hydroxy Benzoic Acid Ethyl ester in Fresh Bamboo Liquid Compound by HPLC

目的建立复方鲜竹沥液中对羟基苯甲酸乙酯的含量测定方法。方法以Lichrospher C18柱（4.6×250mm,5μm）为色谱柱;以A：0.025mol·L-1磷酸二氢钠溶液（磷酸调p H 3.7）-B：甲醇为梯度洗脱;检测波长：258nm;流速：1.0m L·min-1;柱温：35℃。结果对羟基苯甲酸乙酯在0.6915~55.32μg·m L-1范围内呈良好的线性关系（r=0.9999）,平均回收率为96.31%,RSD为1.17%（n=6）。结论本方法简便可行,重现性好,可用于复方鲜竹沥液中对羟基苯甲酸乙酯的含量测定。

OBJECTIVE Establish a determination method of p-hydroxy benzoic acid ethyl ester in fresh bam- boo liquid. METHODS Lichrospher Cls column （4.6 × 250mm, including 5μm） for the chromatographic column; In A ：0. 025mol · L-1 sodium dihydrogen phosphate solution （adjustable pH 3.7 phosphate）-B ：methanol as the gra- dient elution ; Detection wavelength ： 258nm ; Flow rate ： 1.0mL · min -l ; Column temperature 35℃. RESULTS P- hydroxy benzoic acid ethyl ester had a good linear and linear correlation in the range of 0. 6915 ~ 55.32μg · mL-l（ r =0. 9999） ,the average recovery was 96. 31% ,RSD was 1.17% （n =6）. CONCLUSION This method is simple and feasible, and reproducible, and Can be used to compound in fresh bamboo liquid content determination of hydrox- yl benzoic acid ethyl ester.